土耳其收容所犬细小病毒（食肉动物原细小病毒1、食肉动物博卡病毒1和2）的综合研究

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina Pub Date : 2021-05-12 DOI:10.17221/130/2020-vetmed

H. Isidan, T. Turan

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引用次数: 2

摘要

2018年4月，从土耳其锡瓦斯市动物收容所的腹泻犬身上共采集了150份直肠拭子样本。从成年犬身上采集了127份粪便样本，从幼犬身上收集了23份样本。用三套新引物对2型犬细小病毒（食肉动物原细小病毒1）、微小犬病毒（食肉博卡病毒1）和博卡病毒（食肉博卡病毒2）进行了PCR检测。对一些扩增子进行分子测序和分子分析。在127只成年狗中，发现3份粪便样本（2.36%）对食肉动物原细小病毒1呈阳性，5份粪便样本对食肉动物博卡病毒1呈阴性（3.94%），3份样本（2.36%）对食肉病毒博卡病毒2呈阳性。另一方面，在幼犬的粪便样本中，有14/23份（60.87%）对食肉动物原细小病毒1呈阳性，8/23份（34.78%）对食肉病毒1呈阴性，以及6/23份（26.09%）对食肉生物2呈阳性。此外，根据分子分析，我们检测到了食肉动物博卡病毒1和2的两个不同的分支。据我们所知，这是首次在土耳其直接检测到博卡病毒1型和2型食肉动物的报告。

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A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey

A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey.

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来源期刊

Veterinarni Medicina Veterinary Sciences-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.