斯里兰卡皮肤利什曼病患者中发现的利什曼-多诺万体细胞形态学变异

IF 2 Q3 INFECTIOUS DISEASES
A. Murugananthan, Kanchana P. Amarasinghe, G. Rathnaweera, Ponnathurai T. Amalraajan, Rajadurai Arulenthiran
{"title":"斯里兰卡皮肤利什曼病患者中发现的利什曼-多诺万体细胞形态学变异","authors":"A. Murugananthan, Kanchana P. Amarasinghe, G. Rathnaweera, Ponnathurai T. Amalraajan, Rajadurai Arulenthiran","doi":"10.1097/IM9.0000000000000118","DOIUrl":null,"url":null,"abstract":"Abstract Leishmania parasites undergo a dimorphic life cycle. Diagnosis of cutaneous leishmaniasis (CL) is primarily made by the microscopic identification of the amastigote form of the parasite in a smear. Therefore, correct identification of Leishman-Donovan (LD) bodies in smears is crucial for CL confirmation. The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani, which causes CL in Sri Lanka. A total of 125 smears, prepared from patients who met the positive diagnostic criteria of CL, were Giemsa stained and examined microscopically. The density, cellular location and all possible cytomorphological forms of the parasite were documented. A total of 10 different cytomorphological forms of LD bodies were identified. Among them, the round form was observed only intracellularly. The other morphological forms, namely the pear shape, cigarette shape, candle flame form, embryo shape, flagellate form, binary form and rosette form, were found only extracellularly. The spindle form and lens shape were observed in both intracellular and extracellular locations. L. donovani amastigotes have a wide range of morphologies besides their classical forms. Although Leishmania are considered as obligatory intracellular parasites, they manage to survive successfully within the extracellular hostile conditions as well. Therefore, having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"93 - 100"},"PeriodicalIF":2.0000,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cytomorphological Variations of Leishman-Donovan Bodies Found in Cutaneous Leishmaniasis Patients in Sri Lanka\",\"authors\":\"A. Murugananthan, Kanchana P. Amarasinghe, G. Rathnaweera, Ponnathurai T. Amalraajan, Rajadurai Arulenthiran\",\"doi\":\"10.1097/IM9.0000000000000118\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Leishmania parasites undergo a dimorphic life cycle. Diagnosis of cutaneous leishmaniasis (CL) is primarily made by the microscopic identification of the amastigote form of the parasite in a smear. Therefore, correct identification of Leishman-Donovan (LD) bodies in smears is crucial for CL confirmation. The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani, which causes CL in Sri Lanka. A total of 125 smears, prepared from patients who met the positive diagnostic criteria of CL, were Giemsa stained and examined microscopically. The density, cellular location and all possible cytomorphological forms of the parasite were documented. A total of 10 different cytomorphological forms of LD bodies were identified. Among them, the round form was observed only intracellularly. The other morphological forms, namely the pear shape, cigarette shape, candle flame form, embryo shape, flagellate form, binary form and rosette form, were found only extracellularly. The spindle form and lens shape were observed in both intracellular and extracellular locations. L. donovani amastigotes have a wide range of morphologies besides their classical forms. Although Leishmania are considered as obligatory intracellular parasites, they manage to survive successfully within the extracellular hostile conditions as well. Therefore, having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL.\",\"PeriodicalId\":73374,\"journal\":{\"name\":\"Infectious microbes & diseases\",\"volume\":\"5 1\",\"pages\":\"93 - 100\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-05-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infectious microbes & diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1097/IM9.0000000000000118\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectious microbes & diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/IM9.0000000000000118","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

利什曼原虫经历一个二态生命周期。皮肤利什曼病(CL)的诊断主要是通过在涂片中显微镜鉴定寄生虫的无鞭毛体形式。因此,在涂片中正确识别利什曼-多诺万(LD)体对于CL的确认至关重要。本研究的目的是提供LD体不同细胞形态的广泛概述,以提高显微镜检测利什曼原虫的灵敏度,这是导致斯里兰卡CL的原因。从符合CL阳性诊断标准的患者中制备的总共125张涂片,进行吉姆萨染色并进行显微镜检查。记录了寄生虫的密度、细胞位置和所有可能的细胞形态。共鉴定出10种不同的LD细胞形态。其中圆形仅在细胞内可见。梨形、烟形、烛焰形、胚形、鞭毛形、二元形、莲座形等其他形态仅在细胞外发现。在细胞内和细胞外均观察到梭形和晶状体形状。多诺瓦氏无尾线虫除具有经典形态外,还具有广泛的形态特征。虽然利什曼原虫被认为是细胞内的强制性寄生虫,但它们也能在细胞外的恶劣条件下成功生存。因此,对利什曼原虫的不同形态有更广泛的认识可能有助于提高对利什曼原虫的诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytomorphological Variations of Leishman-Donovan Bodies Found in Cutaneous Leishmaniasis Patients in Sri Lanka
Abstract Leishmania parasites undergo a dimorphic life cycle. Diagnosis of cutaneous leishmaniasis (CL) is primarily made by the microscopic identification of the amastigote form of the parasite in a smear. Therefore, correct identification of Leishman-Donovan (LD) bodies in smears is crucial for CL confirmation. The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani, which causes CL in Sri Lanka. A total of 125 smears, prepared from patients who met the positive diagnostic criteria of CL, were Giemsa stained and examined microscopically. The density, cellular location and all possible cytomorphological forms of the parasite were documented. A total of 10 different cytomorphological forms of LD bodies were identified. Among them, the round form was observed only intracellularly. The other morphological forms, namely the pear shape, cigarette shape, candle flame form, embryo shape, flagellate form, binary form and rosette form, were found only extracellularly. The spindle form and lens shape were observed in both intracellular and extracellular locations. L. donovani amastigotes have a wide range of morphologies besides their classical forms. Although Leishmania are considered as obligatory intracellular parasites, they manage to survive successfully within the extracellular hostile conditions as well. Therefore, having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信