{"title":"儿童和青少年严重急性呼吸系统综合征冠状病毒2型抗体的患病率:一项单中心研究","authors":"N. Shlyakhova, L. Rak, N. Shevchenko, T. Holovko","doi":"10.15574/sp.2023.132.62","DOIUrl":null,"url":null,"abstract":"Despite the fact that children with COVID-19 are usually asymptomatic or mild, infection with SARS-CoV-2 is more likely to lead to the development of an antibody response. Understanding humoral immunity to SARS-CoV-2, including in children, is critical for future public health and vaccination strategies. Children with chronic diseases deserve special attention. Purpose - to study the prevalence of positive antibodies to SARS-CoV-2 in children and adolescents who were in paediatric hospital without signs of viral disease to develop future healthcare strategies. Materials and methods. In 32 children aged 6-17 years (14 (43.8%) boys and 16 (56.2%) girls) quantitative determination of IgG antibodies to SARS-CoV-2 in blood serum was carried out by enzyme immunoassay using commercial SARS-CoV-2 S1\\RBD IgG Ab ELISA (TECAN, IBL International GmbH, Germany). Results. The majority (n=27, 84.4%) of children had a positive level of class G antibodies to SARS-CoV-2. 7 (25.9%) children had a positive PCR test in their anamnesis, 6 (18.8%) persons provided information about the infection transmitted by family members. Clinical manifestations characteristic of SARS-CoV-19 were present in 2 (7.4%) children. Parents of 18 (66.7%) children claimed that their children had no symptoms of COVID-19, while family members of 6 (33.3%) children had cases of the disease. All children (n=6, 18.8%) who were vaccinated with two doses of Comirnaty® vaccine had positive IgG antibodies to SARS-CoV-2. Antibodies to SARS-CoV-2 were absent in 5 (15.4%) people, all of them were patients with type 1 diabetes. Conclusions. The study of the prevalence of antibodies to SARS-CoV-2 provides important information about circulating antibodies against SARS-CoV-2 in children and adolescents, in particular those with chronic diseases. The obtained results showed the predominant seropositivity of children, which is formed regardless of the clinical manifestations of the disease. A high level of antibodies under the conditions of revaccination confirms the benefit of revaccination of children to strengthen memory formation. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":34724,"journal":{"name":"Suchasna pediatriia Ukrayina","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of antibodies to SARS-COV-2 in children and adolescents: a single-center study\",\"authors\":\"N. Shlyakhova, L. Rak, N. Shevchenko, T. Holovko\",\"doi\":\"10.15574/sp.2023.132.62\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Despite the fact that children with COVID-19 are usually asymptomatic or mild, infection with SARS-CoV-2 is more likely to lead to the development of an antibody response. Understanding humoral immunity to SARS-CoV-2, including in children, is critical for future public health and vaccination strategies. Children with chronic diseases deserve special attention. Purpose - to study the prevalence of positive antibodies to SARS-CoV-2 in children and adolescents who were in paediatric hospital without signs of viral disease to develop future healthcare strategies. Materials and methods. In 32 children aged 6-17 years (14 (43.8%) boys and 16 (56.2%) girls) quantitative determination of IgG antibodies to SARS-CoV-2 in blood serum was carried out by enzyme immunoassay using commercial SARS-CoV-2 S1\\\\RBD IgG Ab ELISA (TECAN, IBL International GmbH, Germany). Results. The majority (n=27, 84.4%) of children had a positive level of class G antibodies to SARS-CoV-2. 7 (25.9%) children had a positive PCR test in their anamnesis, 6 (18.8%) persons provided information about the infection transmitted by family members. Clinical manifestations characteristic of SARS-CoV-19 were present in 2 (7.4%) children. Parents of 18 (66.7%) children claimed that their children had no symptoms of COVID-19, while family members of 6 (33.3%) children had cases of the disease. All children (n=6, 18.8%) who were vaccinated with two doses of Comirnaty® vaccine had positive IgG antibodies to SARS-CoV-2. Antibodies to SARS-CoV-2 were absent in 5 (15.4%) people, all of them were patients with type 1 diabetes. Conclusions. The study of the prevalence of antibodies to SARS-CoV-2 provides important information about circulating antibodies against SARS-CoV-2 in children and adolescents, in particular those with chronic diseases. The obtained results showed the predominant seropositivity of children, which is formed regardless of the clinical manifestations of the disease. A high level of antibodies under the conditions of revaccination confirms the benefit of revaccination of children to strengthen memory formation. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.\",\"PeriodicalId\":34724,\"journal\":{\"name\":\"Suchasna pediatriia Ukrayina\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Suchasna pediatriia Ukrayina\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15574/sp.2023.132.62\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Suchasna pediatriia Ukrayina","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15574/sp.2023.132.62","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
尽管新冠肺炎儿童通常无症状或轻微,但感染SARS-CoV-2更有可能导致抗体反应的发展。了解包括儿童在内的严重急性呼吸系统综合征冠状病毒2型的体液免疫,对未来的公共卫生和疫苗接种策略至关重要。患有慢性病的儿童值得特别关注。目的-研究在儿科医院没有病毒性疾病迹象的儿童和青少年中严重急性呼吸系统综合征冠状病毒2型阳性抗体的流行率,以制定未来的医疗策略。材料和方法。在32名6-17岁的儿童(14名(43.8%)男孩和16名(56.2%)女孩)中,使用商业化的严重急性呼吸系统综合征冠状病毒2型S1\RBD IgG Ab ELISA(TECAN,IBL International GmbH,德国)通过酶免疫测定法对血清中严重急性呼吸系统冠状病毒2型IgG抗体进行定量测定。后果大多数(n=27,84.4%)儿童的严重急性呼吸系统综合征冠状病毒2型G类抗体水平呈阳性。7名(25.9%)儿童的记忆中PCR检测呈阳性,6名(18.8%)儿童提供了由家庭成员传播的感染信息。2例(7.4%)儿童出现严重急性呼吸系统综合征冠状病毒-19的临床表现。18名(66.7%)儿童的父母声称他们的孩子没有新冠肺炎症状,而6名(33.3%)儿童的家庭成员有新冠肺炎病例。所有接种了两剂Comirnaty®疫苗的儿童(n=6,18.8%)的严重急性呼吸系统综合征冠状病毒2型IgG抗体均呈阳性。5人(15.4%)没有严重急性呼吸系统综合征冠状病毒2型抗体,他们都是1型糖尿病患者。结论。对严重急性呼吸系统综合征冠状病毒2型抗体流行率的研究提供了有关儿童和青少年,特别是患有慢性病的儿童和青少年中循环的严重急性呼吸系综合征病毒2型抗体的重要信息。所获得的结果显示,无论疾病的临床表现如何,都会形成儿童的主要血清阳性。在重新接种疫苗的条件下,高水平的抗体证实了儿童重新接种疫苗对加强记忆形成的益处。这项研究是根据《赫尔辛基宣言》的原则进行的。参与机构的地方伦理委员会批准了该研究方案。进行研究获得了患者的知情同意。提交人没有宣布任何利益冲突。
Prevalence of antibodies to SARS-COV-2 in children and adolescents: a single-center study
Despite the fact that children with COVID-19 are usually asymptomatic or mild, infection with SARS-CoV-2 is more likely to lead to the development of an antibody response. Understanding humoral immunity to SARS-CoV-2, including in children, is critical for future public health and vaccination strategies. Children with chronic diseases deserve special attention. Purpose - to study the prevalence of positive antibodies to SARS-CoV-2 in children and adolescents who were in paediatric hospital without signs of viral disease to develop future healthcare strategies. Materials and methods. In 32 children aged 6-17 years (14 (43.8%) boys and 16 (56.2%) girls) quantitative determination of IgG antibodies to SARS-CoV-2 in blood serum was carried out by enzyme immunoassay using commercial SARS-CoV-2 S1\RBD IgG Ab ELISA (TECAN, IBL International GmbH, Germany). Results. The majority (n=27, 84.4%) of children had a positive level of class G antibodies to SARS-CoV-2. 7 (25.9%) children had a positive PCR test in their anamnesis, 6 (18.8%) persons provided information about the infection transmitted by family members. Clinical manifestations characteristic of SARS-CoV-19 were present in 2 (7.4%) children. Parents of 18 (66.7%) children claimed that their children had no symptoms of COVID-19, while family members of 6 (33.3%) children had cases of the disease. All children (n=6, 18.8%) who were vaccinated with two doses of Comirnaty® vaccine had positive IgG antibodies to SARS-CoV-2. Antibodies to SARS-CoV-2 were absent in 5 (15.4%) people, all of them were patients with type 1 diabetes. Conclusions. The study of the prevalence of antibodies to SARS-CoV-2 provides important information about circulating antibodies against SARS-CoV-2 in children and adolescents, in particular those with chronic diseases. The obtained results showed the predominant seropositivity of children, which is formed regardless of the clinical manifestations of the disease. A high level of antibodies under the conditions of revaccination confirms the benefit of revaccination of children to strengthen memory formation. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.