生物测定指导黄精对异烟肼和利福平肝毒性的保护作用

Q4 Pharmacology, Toxicology and Pharmaceutics
P. Grover, R. Ghai, K. Nagarajan, Vinay V. Kumar, R. Goel, Charanpreet Kaur, Reenu Chauhan
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引用次数: 0

摘要

本研究旨在观察黄精乙醇水提取物对抗结核药物性肝损伤的保护作用。将红车轴草根状茎粉碎,溶解在各种溶剂中(按极性顺序),然后测试植物化学物质。基于他们的发现,使用Soxhlet方法利用乙醇-水混合物(50:50)进行大量提取。动物研究的剂量是通过急性毒性试验确定的。根状茎乙醇水提取物(50:50)在Wistar大鼠中以每天200mg kg-1和400mg kg-1 p.o.的剂量测定肝保护潜力。检查血液样本中的生化标志物SGOT、SGPT、ALP、总胆红素和白蛋白。还进行了肝脏的组织病理学研究,随后进行了体外抗氧化研究。同时,对提取物进行LCMS表征。如血液生物标志物值的显著变化和体外抗氧化研究所示,在200 mg kg-1和400 mg kg-1剂量下,红车轴草提取物对INH+RIF诱导的肝毒性均表现出显著的肝保护活性,且呈剂量依赖性。组织病理学研究表明,200 mg kg-1和400 mg kg-1的紫车轴草治疗通过减少出血和炎症浸润,表现出肝脏结构和门脉系统的再生。LC-MS分析表明,蛇纹石、5-羟甲基糠醛和头孢噻嗪为有效成分。可以推断,红车轴草的水乙醇提取物可以保护肝脏免受TB诱导的毒性,这种保护可能是由于其活性植物成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BIOASSAY GUIDED HEPATOPROTECTIVE ACTIVITY OF POLYGONATUM CIRRHIFOLIUM AGAINST ISONIAZID AND RIFAMPICIN INDUCED HEPATOTOXICITY IN RATS
The present investigation was performed to examine the hepatoprotective effect of the aqueous ethanolic extract of Polygonatum cirrhifolium in antitubercular drug-induced liver damage. P. cirrhifolium rhizomes were crushed, dissolved in various solvents (in order of polarity), and then tested for phytochemicals. Based on their findings, mass extraction utilizing the ethanol-water mixture (50: 50) was carried out using the Soxhlet method. The doses for animal research were established through acute toxicity tests. The hepatoprotective potential of aqueous ethanolic extract (50:50) of rhizomes was determined in Wistar rats at doses of 200 mg kg-1 and 400 mg kg-1 p.o. per day. Blood samples were examined for the biochemical markers SGOT, SGPT, ALP, total bilirubin, and albumin. Histopathology of the liver was also conducted followed by in vitro anti-oxidant studies. Simultaneously, the extract was subjected to LCMS characterization. P. cirrhifolium extract at both the doses 200 mg kg-1 and 400 mg kg-1 has shown significant hepatoprotective activity against hepatotoxicity induced by INH+ RIF in a dose-dependent manner, as depicted by the significant changes in the values of blood biomarkers and in vitro anti-oxidant studies. Histopathological studies showed that the treatment with 200 mg kg-1 and 400 mg kg-1 of P. cirrhifolium exhibited regeneration of liver architecture and portal system by reducing the haemorrhage and inflammatory infiltrate. LC-MS characterization showed serpentine, 5-hydroxy methylfurfural and cephalotaxine as active constituents. It can be inferred that hydroethanolic extract of P. cirrhifolium protects the liver from anti-TB induced toxicity and this protection could be due to the active phytoconstituents.
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来源期刊
INDIAN DRUGS
INDIAN DRUGS Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.30
自引率
0.00%
发文量
98
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