链脲佐菌素诱导的糖尿病大鼠在烟酸氧亚乙基二膦酸锗酸盐(MIGU-4)治疗条件下视网膜的神经退行性变化

Q4 Medicine
В.Й. Кресюн, Нора Джавад Аль-Надаві
{"title":"链脲佐菌素诱导的糖尿病大鼠在烟酸氧亚乙基二膦酸锗酸盐(MIGU-4)治疗条件下视网膜的神经退行性变化","authors":"В.Й. Кресюн, Нора Джавад Аль-Надаві","doi":"10.31288/oftalmolzh202345560","DOIUrl":null,"url":null,"abstract":"Background: Diabetes is accompanied by neurodegenerative changes in the retina. The determination of retinal function parameters under conditions of experimental diabetes may allow for the development of new approaches to pharmacological vision correction.\n\nPurpose: To assess changes in the metabolic (lipid peroxidation and antioxidant defence) and functional characteristics (electroretinography (ERG)) of the retina in the rat with streptozotocin (STZ)-induced diabetes, treated with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) and a reference drug, diazepam.\n\nMaterial and Methods: Diabetes was induced intraperitoneally (i.p.) in Wistar male rats by streptozotocin (STZ 65 mg/kg). Four weeks thereafter, the rats received a two-week course of a daily dose of MIGU-4 (5.0 mg/kg or 25.0 mg/kg) or diazepam (0.5 mg/kg or 1.5 mg/kg). The electroretinogram (ERG) was obtained, the animals were euthanized and retinal malondialdehyde (MDA) levels and superoxide dismutase (SOD) and catalase (CAT) activity were determined.\n\nResults: In rats with untreated STZ-induced diabetes, the retinal MDA level was 3.71 times increased and CAT activity was 35% lower, compared to controls, whereas the SOD activity was half of the activity found in controls (р less 0.05). In diabetic animals treated with MIGU-4 i.p. at a 25.0-mg/kg daily dose, the retinal MDA level was 63.2% lower, whereas the CAT activity and SOD activity were 41.9% and 27.6%, respectively, higher, than in untreated diabetic rats (р less 0.05). In diabetic animals treated with diazepam i.p. at a 1.5-mg/kg daily dose, the retinal MDA level was 59.2% lower, whereas the CAT activity and SOD activity were 44.4% and 32.1%, respectively, higher, than in untreated diabetic rats (р less 0.05). In untreated diabetic rats, the ERG b-wave amplitude was 39.2% lower, ERG a-wave and b-wave latencies, 23.4% and 14.0%, respectively, higher, and the a-wave amplitude recovery rate, 38.8% lower than in controls (р less 0.05). The use of a 25.0-mg/kg daily dose of MIGU-4 resulted in a 29.7% and 33.9%, respectively, increase in the ERG b-wave amplitude and the a-wave amplitude recovery rate compared to untreated diabetic rats (р less 0.05). The use of a 1.5-mg/kg daily dose of diazepam resulted in a 25.0% and 30.0%, respectively, increase in the above ERG parameters compared to untreated diabetic rats (р less 0.05). In untreated diabetic rats, the number of squares crossed (SK) was 29.4% lower (р less 0.05); the number of central squares crossed (CSC), 52.1% lower (р less 0.05); the number of upright postures (UP), 34.2% lower; and the defecation bolus number, 32.4% higher than in controls (р less 0.05). After MIGU-4 (25-mg/kg) withdrawal, there were no more differences in SK, CSC, UP and defecation bolus numbers between treated diabetic rats and controls. In addition, the number of CSC was 4.27 times lower than in controls, and half of that in untreated diabetic animals (р less 0.05). In diabetic rats treated with diazepam, the number of UP was 2.56 times lower than in controls (р less 0.05), and 40.7% lower than in untreated diabetic rats (р less 0.05), whereas the defecation bolus number was 40.5% higher than in controls (р less 0.05).\n\nConclusion: STZ-induced diabetes is accompanied by an impairment of the oxidant/ antioxidant balance in the retina with an increase in retinal MDA, decrease in retinal SOD and CAT activity, and ERG abnormalities such as reduced ERG amplitude and increased ERG latency. A course treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) i.p. at a daily dose of 25.0 mg/kg provides for a decrease in retinal MDA and an increase in retinal SOD and CAT activity and ERG wave amplitude The withdrawal of a two-week treatment with MIGU-4 i.p. at a daily dose of 25.0 mg/kg decreased open-field anxiety-like behaviors, whereas the withdrawal of a two-week treatment with diazepam i.p. at a daily dose of 1.5 mg/kg increased open-field anxiety-like behaviors. The corrective effect of treatment with MIGU-4 corresponds to the corrective effect of treatment with diazepam.","PeriodicalId":19419,"journal":{"name":"Oftalmologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Neurodegenrative changes in retina of rats with streptozotocin-induced diabetes under conditions of treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4)\",\"authors\":\"В.Й. Кресюн, Нора Джавад Аль-Надаві\",\"doi\":\"10.31288/oftalmolzh202345560\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Diabetes is accompanied by neurodegenerative changes in the retina. The determination of retinal function parameters under conditions of experimental diabetes may allow for the development of new approaches to pharmacological vision correction.\\n\\nPurpose: To assess changes in the metabolic (lipid peroxidation and antioxidant defence) and functional characteristics (electroretinography (ERG)) of the retina in the rat with streptozotocin (STZ)-induced diabetes, treated with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) and a reference drug, diazepam.\\n\\nMaterial and Methods: Diabetes was induced intraperitoneally (i.p.) in Wistar male rats by streptozotocin (STZ 65 mg/kg). Four weeks thereafter, the rats received a two-week course of a daily dose of MIGU-4 (5.0 mg/kg or 25.0 mg/kg) or diazepam (0.5 mg/kg or 1.5 mg/kg). The electroretinogram (ERG) was obtained, the animals were euthanized and retinal malondialdehyde (MDA) levels and superoxide dismutase (SOD) and catalase (CAT) activity were determined.\\n\\nResults: In rats with untreated STZ-induced diabetes, the retinal MDA level was 3.71 times increased and CAT activity was 35% lower, compared to controls, whereas the SOD activity was half of the activity found in controls (р less 0.05). In diabetic animals treated with MIGU-4 i.p. at a 25.0-mg/kg daily dose, the retinal MDA level was 63.2% lower, whereas the CAT activity and SOD activity were 41.9% and 27.6%, respectively, higher, than in untreated diabetic rats (р less 0.05). In diabetic animals treated with diazepam i.p. at a 1.5-mg/kg daily dose, the retinal MDA level was 59.2% lower, whereas the CAT activity and SOD activity were 44.4% and 32.1%, respectively, higher, than in untreated diabetic rats (р less 0.05). In untreated diabetic rats, the ERG b-wave amplitude was 39.2% lower, ERG a-wave and b-wave latencies, 23.4% and 14.0%, respectively, higher, and the a-wave amplitude recovery rate, 38.8% lower than in controls (р less 0.05). The use of a 25.0-mg/kg daily dose of MIGU-4 resulted in a 29.7% and 33.9%, respectively, increase in the ERG b-wave amplitude and the a-wave amplitude recovery rate compared to untreated diabetic rats (р less 0.05). The use of a 1.5-mg/kg daily dose of diazepam resulted in a 25.0% and 30.0%, respectively, increase in the above ERG parameters compared to untreated diabetic rats (р less 0.05). In untreated diabetic rats, the number of squares crossed (SK) was 29.4% lower (р less 0.05); the number of central squares crossed (CSC), 52.1% lower (р less 0.05); the number of upright postures (UP), 34.2% lower; and the defecation bolus number, 32.4% higher than in controls (р less 0.05). After MIGU-4 (25-mg/kg) withdrawal, there were no more differences in SK, CSC, UP and defecation bolus numbers between treated diabetic rats and controls. In addition, the number of CSC was 4.27 times lower than in controls, and half of that in untreated diabetic animals (р less 0.05). In diabetic rats treated with diazepam, the number of UP was 2.56 times lower than in controls (р less 0.05), and 40.7% lower than in untreated diabetic rats (р less 0.05), whereas the defecation bolus number was 40.5% higher than in controls (р less 0.05).\\n\\nConclusion: STZ-induced diabetes is accompanied by an impairment of the oxidant/ antioxidant balance in the retina with an increase in retinal MDA, decrease in retinal SOD and CAT activity, and ERG abnormalities such as reduced ERG amplitude and increased ERG latency. A course treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) i.p. at a daily dose of 25.0 mg/kg provides for a decrease in retinal MDA and an increase in retinal SOD and CAT activity and ERG wave amplitude The withdrawal of a two-week treatment with MIGU-4 i.p. at a daily dose of 25.0 mg/kg decreased open-field anxiety-like behaviors, whereas the withdrawal of a two-week treatment with diazepam i.p. at a daily dose of 1.5 mg/kg increased open-field anxiety-like behaviors. The corrective effect of treatment with MIGU-4 corresponds to the corrective effect of treatment with diazepam.\",\"PeriodicalId\":19419,\"journal\":{\"name\":\"Oftalmologicheskii zhurnal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oftalmologicheskii zhurnal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31288/oftalmolzh202345560\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oftalmologicheskii zhurnal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31288/oftalmolzh202345560","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

背景:糖尿病伴视网膜神经退行性改变。在实验性糖尿病条件下视网膜功能参数的测定可能允许开发新的药物视力矫正方法。目的:评价链脲佐菌素(STZ)诱导的糖尿病大鼠在烟酸-氧-乙基-二膦酸德酸酯(MIGU-4)和参比药物地西泮治疗后视网膜代谢(脂质过氧化和抗氧化防御)和功能特征(视网膜电图(ERG))的变化。材料与方法:采用链脲佐菌素(STZ 65 mg/kg)腹腔注射诱导Wistar雄性大鼠糖尿病。四周后,大鼠接受为期两周的每日剂量的MIGU-4 (5.0 mg/kg或25.0 mg/kg)或地西泮(0.5 mg/kg或1.5 mg/kg)疗程。取视网膜电图(ERG),对大鼠实施安乐死,测定视网膜丙二醛(MDA)水平、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。结果:与对照组相比,未经stz治疗的糖尿病大鼠视网膜MDA水平升高3.71倍,CAT活性降低35%,SOD活性为对照组的一半(p < 0.05)。25.0 mg/kg日剂量MIGU-4组糖尿病大鼠视网膜MDA水平比未处理组降低63.2%,CAT活性和SOD活性分别提高41.9%和27.6% (p < 0.05)。日给药1.5 mg/kg地西泮组大鼠视网膜MDA水平比未给药组低59.2%,CAT活性和SOD活性分别比未给药组高44.4%和32.1% (p < 0.05)。与对照组相比,未经治疗的糖尿病大鼠ERG b波振幅降低39.2%,ERG a波和b波潜伏期分别升高23.4%和14.0%,a波振幅恢复率降低38.8% (p < 0.05)。使用25.0 mg/kg日剂量的MIGU-4可使糖尿病大鼠ERG b波振幅和a波振幅恢复率分别提高29.7%和33.9% (p < 0.05)。与未给药的糖尿病大鼠相比,每天给药1.5 mg/kg的地西泮使上述ERG参数分别增加25.0%和30.0% (p < 0.05)。未经治疗的糖尿病大鼠,交叉方框数(SK)降低29.4% (p < 0.05);交叉中心方数(CSC)降低52.1% (< 0.05);直立姿势(UP)次数减少34.2%;排便丸数较对照组增加32.4% (p < 0.05)。停用MIGU-4 (25 mg/kg)后,糖尿病大鼠的SK、CSC、UP和排便量与对照组无明显差异。此外,CSC数量比对照组低4.27倍,为未治疗糖尿病动物的一半(p < 0.05)。地西泮组糖尿病大鼠的UP次数比对照组低2.56倍(p < 0.05),比未治疗组低40.7% (p < 0.05),排便量比对照组高40.5% (p < 0.05)。结论:stz诱导的糖尿病伴有视网膜氧化/抗氧化平衡受损,表现为视网膜MDA升高,视网膜SOD和CAT活性降低,ERG振幅降低,ERG潜伏期增加等异常。课程治疗niacin-oxy-ethylidene-diphosphonate锗酸盐(MIGU-4) ip每日剂量为25.0毫克/公斤提供了降低视网膜MDA和增加视网膜SOD和CAT活性和ERG波振幅的回避,为期两周的治疗MIGU-4 ip每日剂量为25.0毫克/公斤减少开放田地焦虑行为,而撤出与安定ip为期两周的治疗剂量为1.5毫克/公斤增加开放田地焦虑行为。MIGU-4治疗的矫正效果与地西泮治疗的矫正效果相对应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neurodegenrative changes in retina of rats with streptozotocin-induced diabetes under conditions of treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4)
Background: Diabetes is accompanied by neurodegenerative changes in the retina. The determination of retinal function parameters under conditions of experimental diabetes may allow for the development of new approaches to pharmacological vision correction. Purpose: To assess changes in the metabolic (lipid peroxidation and antioxidant defence) and functional characteristics (electroretinography (ERG)) of the retina in the rat with streptozotocin (STZ)-induced diabetes, treated with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) and a reference drug, diazepam. Material and Methods: Diabetes was induced intraperitoneally (i.p.) in Wistar male rats by streptozotocin (STZ 65 mg/kg). Four weeks thereafter, the rats received a two-week course of a daily dose of MIGU-4 (5.0 mg/kg or 25.0 mg/kg) or diazepam (0.5 mg/kg or 1.5 mg/kg). The electroretinogram (ERG) was obtained, the animals were euthanized and retinal malondialdehyde (MDA) levels and superoxide dismutase (SOD) and catalase (CAT) activity were determined. Results: In rats with untreated STZ-induced diabetes, the retinal MDA level was 3.71 times increased and CAT activity was 35% lower, compared to controls, whereas the SOD activity was half of the activity found in controls (р less 0.05). In diabetic animals treated with MIGU-4 i.p. at a 25.0-mg/kg daily dose, the retinal MDA level was 63.2% lower, whereas the CAT activity and SOD activity were 41.9% and 27.6%, respectively, higher, than in untreated diabetic rats (р less 0.05). In diabetic animals treated with diazepam i.p. at a 1.5-mg/kg daily dose, the retinal MDA level was 59.2% lower, whereas the CAT activity and SOD activity were 44.4% and 32.1%, respectively, higher, than in untreated diabetic rats (р less 0.05). In untreated diabetic rats, the ERG b-wave amplitude was 39.2% lower, ERG a-wave and b-wave latencies, 23.4% and 14.0%, respectively, higher, and the a-wave amplitude recovery rate, 38.8% lower than in controls (р less 0.05). The use of a 25.0-mg/kg daily dose of MIGU-4 resulted in a 29.7% and 33.9%, respectively, increase in the ERG b-wave amplitude and the a-wave amplitude recovery rate compared to untreated diabetic rats (р less 0.05). The use of a 1.5-mg/kg daily dose of diazepam resulted in a 25.0% and 30.0%, respectively, increase in the above ERG parameters compared to untreated diabetic rats (р less 0.05). In untreated diabetic rats, the number of squares crossed (SK) was 29.4% lower (р less 0.05); the number of central squares crossed (CSC), 52.1% lower (р less 0.05); the number of upright postures (UP), 34.2% lower; and the defecation bolus number, 32.4% higher than in controls (р less 0.05). After MIGU-4 (25-mg/kg) withdrawal, there were no more differences in SK, CSC, UP and defecation bolus numbers between treated diabetic rats and controls. In addition, the number of CSC was 4.27 times lower than in controls, and half of that in untreated diabetic animals (р less 0.05). In diabetic rats treated with diazepam, the number of UP was 2.56 times lower than in controls (р less 0.05), and 40.7% lower than in untreated diabetic rats (р less 0.05), whereas the defecation bolus number was 40.5% higher than in controls (р less 0.05). Conclusion: STZ-induced diabetes is accompanied by an impairment of the oxidant/ antioxidant balance in the retina with an increase in retinal MDA, decrease in retinal SOD and CAT activity, and ERG abnormalities such as reduced ERG amplitude and increased ERG latency. A course treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) i.p. at a daily dose of 25.0 mg/kg provides for a decrease in retinal MDA and an increase in retinal SOD and CAT activity and ERG wave amplitude The withdrawal of a two-week treatment with MIGU-4 i.p. at a daily dose of 25.0 mg/kg decreased open-field anxiety-like behaviors, whereas the withdrawal of a two-week treatment with diazepam i.p. at a daily dose of 1.5 mg/kg increased open-field anxiety-like behaviors. The corrective effect of treatment with MIGU-4 corresponds to the corrective effect of treatment with diazepam.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Oftalmologicheskii zhurnal
Oftalmologicheskii zhurnal Medicine-Ophthalmology
CiteScore
0.40
自引率
0.00%
发文量
71
期刊介绍: Journal of Ophthalmology (Ukraine) contains articles on eye diseases diagnosis and treatment, eye care, eye diseases prevention, history of ophthalmology, organization of eye care to population, technical equipment problems. It is committed to publishing original scientific researches and review articles on theory and practice of Ukrainian and foreign ophthalmology, cases, reviews, inventions, historical and medical reviews. It contains peer-reviews of books on ophthalmology, articles on activity of ophthalmologic societies, congresses and conferences chronicles. Journal is designed to ophthalmologists and scientific researchers in the field of vision physiology and eye diseases clinic.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信