Oxidative Damages of Two Neonicotinoid Pesticides to Arthrospira platensis (Gomont)

In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, MDA, H2O2 and Proline) were determined in order to understand the effects of Thiacloprid and Imidacloprid on Arthrospira platensis Gomont. Both Imidacloprid and Thiacloprid applications showed significant reductions in growth rate and chlorophyll-a content of A. platensis cultures with dose-dependent manner when the days and concentrations compared each other. SOD activity significantly decreased in the Imidacloprid application while Thiacloprid caused a significant increase only at 75 µg mL-1 concentration. APX activity significantly increased in the Imidacloprid and Thiacloprid applications at 50 µg mL-1 and 35 µg mL-1 concentrations, respectively. Imidacloprid treatment increased GR activity at 20 and 30 µg mL-1 concentrations while GR activity increased at 15, 25 and 35 µg mL-1 Thiacloprid concentrations. MDA content of A. platensis cultures did not show alterations neither with Imidacloprid nor with Thiacloprid applications. The H2O2 content did not change at all Imidacloprid concentrations. Although the H2O2 content showed a decrease at 15 µg mL-1 concentraiton, it displayed increase at 45 and 75 µg mL-1 Thiacloprid concentrations. Free proline contents increased in the Imidacloprid and Thiacloprid applications at 100 µg mL-1 and 75 µg mL-1 concentrations, respectively. It is obvious that these neonicotinoid pesticides caused to oxidative stress in A. platensis cells.