二倍体和四倍体（自体和异源四倍体）西瓜形态特征的比较研究

IF 2.2 4区农林科学 Q2 HORTICULTURE

Folia Horticulturae Pub Date : 2023-03-30 DOI:10.2478/fhort-2023-0003

A. Aydin, H. Yetişir

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引用次数: 0

摘要

摘要本研究通过气孔观察和流式细胞术分析，测定了0.05%秋水仙碱对第一真叶期幼苗多倍体的倍性水平。根据气孔观察和流式细胞仪分析，在开发多倍体西瓜砧木的研究中，植株的存活率为77%，多倍体化率分别为11%和3%。根据流式细胞术的结果，确定了22种多倍体基因型。开发了Citrullus基因型的Auto-（12）和同素异形体（10），并与二倍体、商业砧木（RS841，‘Argentario’）和西瓜品种（‘Crimson Tide’）进行了水培比较，测定了它们的植株生长性能。在水培培养基中培养21天，测定了假定的四倍体及其二倍体对照的营养生长性能。结果表明，与二倍体对照相比，同源四倍体和异源四倍体的植物生物量分别增加了100%和156%。

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A comparative study of morphological characteristics in diploid and tetraploid (auto and allotetraploids) Citrullus genotypes

ABSTRACT In this study, ploidy levels were determined by stomatal observations and flow cytometry analysis of plants polyploidised by the application of 0.05% colchicine to seedlings at the first true leaf stage. In the study of developing polyploid watermelon rootstocks, the survival rate of the plants was 77%, and the polyploidisation rates were 11% and 3% according to stomatal observations and flow cytometry analysis, respectively. According to the results of flow cytometry, 22 polyploid genotypes were determined. Auto- (12) and allotetraploids (10) of Citrullus genotypes were developed, and their plant growth performance was determined in hydroponic culture in comparison with diploids, commercial rootstocks (RS841, ‘Argentario’) and watermelon cultivar (‘Crimson Tide’). Putative tetraploids and their diploid controls were grown in hydroponic culture for 21 days, and their vegetative growth performances were determined. The results showed that the increases in plant biomass depending on polyploidisation were 100% in autotetraploids and 156% in allotetraploids as compared to diploid controls.

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来源期刊

Folia Horticulturae Agricultural and Biological Sciences-Horticulture

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.