摩洛哥利什曼病焦点植物Sefrou提取物对Promastigote期多种利什曼原虫的杀利什曼活性研究

Q4 Medicine
I. Zeouk, A. Et-Touys, M. Balouiri, H. Fellah, A. E. Lalami, K. Bekhti
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引用次数: 3

摘要

据世界卫生组织称,利什曼病仍然是一个主要的全球公共卫生问题。位于摩洛哥中部的塞弗鲁省是皮肤利什曼病的重镇。本研究旨在评价当地居民常用的小檗属、山楂属、鼠尾草属、麻黄属和薰衣草属植物的抗利什曼病潜力。采用四氮唑基比色法(MTT)测定甲醇提取物对热带利什曼原虫、大利什曼原虫和幼年利什曼原虫的抑菌作用。采用Folin-Ciocalteu试剂、氯化铝溶液和醋酸钾溶液分别测定各提取物的总酚和总黄酮含量。与对照葡聚糖®相比,该植物提取物具有抗利什曼原虫活性,其IC50(最大半数抑制浓度)为100 ~ 1000 μg/mL。其中,小檗对大利什曼原虫(IC50 = 394.40±3.02 μg/ml)的抑制效果最好,而首次报道的深草对婴儿利什曼原虫(IC50 = 490.84±3.15 μg/ml)的抑制效果最好。热带利什曼原虫对5种提取物均表现出相同的敏感性(平均IC50 = 540±11.20 μg/mL)。总酚含量较高forCrataegus oxyacanthaandCistus salviifolius(140.67±3.17μg情商没食子酸(GA) /毫克的提取(E)和133.83±9.03μg eq GA /毫克的E),而类黄酮高forCistus salviifoliusandLavandula dentata(57.92±2.46μg eq槲皮素(是)/毫克的提取(E)和41.53±1.74μg eq, /毫克的E)。所有在场的检测提取一些有前途的方面可能治愈皮肤利什曼病在摩洛哥的中心;需要进一步的生物引导分析来分离这些组分和生物活性分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Leishmanicidal Activity of Plant Extracts from Sefrou, a Moroccan Focus of Leishmaniasis, against Various Leishmania Parasites in the Promastigote Stage
According to the World Health Organization, leishmaniasis remains a major worldwide public health problem. The province of Sefrou located in the center of Morocco is a focus of cutaneous leishmaniasis. The present study aims at evaluating the antileishmanial potential of Berberis sp.,Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima and Lavandula dentatafrequently used by the local population. Methanolic extracts were tested against the promastigote form ofLeishmania tropica, Leishmania majorandLeishmania infantumusing tetrazolium-based colorimetric (MTT) assay. The total phenol and flavonoids content of all extracts were determined using the Folin–Ciocalteu reagent, aluminum chloride, and potassium acetate solutions respectively. The plant extracts exhibited antileishmanial activity with variability depending on the tested strain and the plant species compared to Glucantime® used as control (IC50 (the half maximal inhibitory concentration) > 1,000 μg/mL). The best inhibition was observed with Berberis sp., againstLeishmania major(IC50 = 394.40 ± 3.02 μg/ml), andEphedra altissima(reported for the first time) againstLeishmania infantum(IC50 = 490.84 ± 3.15 μg/mL).Leishmania tropicahas shown the same sensitivity behavior toward the five extracts (in average IC50 = 540 ± 11.20 μg/mL). The total phenolic content was higher forCrataegus oxyacanthaandCistus salviifolius(140.67 ± 3.17 μg eq Gallic Acid (GA)/ mg of Extract (E) and 133.83 ± 9.03 μg eq GA/mg of E respectively), while flavonoid was higher forCistus salviifoliusandLavandula dentata(57.92 ± 2.46 μg eq Quercetin (Que)/ mg of Extract (E) and 41.53 ± 1.74 μg eq Que/mg of E). All the tested extracts present some promising aspects that may cure cutaneous leishmaniasis in the center of Morocco; further bioguided assays are needed to isolate the fractions and the bioactive molecule.
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来源期刊
Phytotherapie
Phytotherapie Medicine-Complementary and Alternative Medicine
CiteScore
0.90
自引率
0.00%
发文量
25
期刊介绍: La phytothérapie fait partie de votre pratique quotidienne : la revue PHYTOTHÉRAPIE est outil de référence dont vous avez besoin. Ce est la seule revue francophone scientifique de formation médicale continue sur ce domaine. La revue PHYTOTHÉRAPIE fait le lien entre le monde de la recherche et les praticiens qui sont sur le terrain.
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