Syeda Maryam Zahid , Muhammad Farooq , Memona Yasmin , Muhammad Qasim Aslam , Shahid Mansoor , Imran Amin
{"title":"选择性剪接在棉花粉变应原(Ole e 1) P19963蛋白调控中起着至关重要的作用","authors":"Syeda Maryam Zahid , Muhammad Farooq , Memona Yasmin , Muhammad Qasim Aslam , Shahid Mansoor , Imran Amin","doi":"10.1016/j.plgene.2022.100362","DOIUrl":null,"url":null,"abstract":"<div><p><span>Alternative splicing regulation (AS) is an important post-transcriptional gene regulatory mechanism for increasing the complexity and diversity of transcriptomes<span><span> and subsequently proteomes. In this study, we performed </span>transcriptomic analysis of </span></span><span><em>Gossypium</em><em> arboreum</em></span> to explore the process of alternative splicing regulation in pollen allergen (<em>Ole e 1</em><span>) P19963 protein. The P19963 causes skin allergies because of its acidic properties. The P19963 protein was observed to undergo intron retention at seedling stage (IR); a type of alternative splicing regulation events when intron is not spliced out after the action of splicing machinery. Experimental validation using RT-PCR and sanger sequencing also proved retention of intronic region in the mature mRNA transcript of P19963 protein. We observed that even if this gene was expressing at later stages of plant development i.e., pollen producing stage, but due to alternative splicing it became disrupted at the seedling stage. The non-functionality of protein can be beneficial or harmful but in our case as the gene is having IR and becomes non-functional, it is beneficial as the pollen allergies and skin allergies can be minimized at this stage or not required at all.</span></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alternative splicing plays a vital role in regulating pollen allergen (Ole e 1) P19963 protein in Gossypium arboreum\",\"authors\":\"Syeda Maryam Zahid , Muhammad Farooq , Memona Yasmin , Muhammad Qasim Aslam , Shahid Mansoor , Imran Amin\",\"doi\":\"10.1016/j.plgene.2022.100362\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Alternative splicing regulation (AS) is an important post-transcriptional gene regulatory mechanism for increasing the complexity and diversity of transcriptomes<span><span> and subsequently proteomes. In this study, we performed </span>transcriptomic analysis of </span></span><span><em>Gossypium</em><em> arboreum</em></span> to explore the process of alternative splicing regulation in pollen allergen (<em>Ole e 1</em><span>) P19963 protein. The P19963 causes skin allergies because of its acidic properties. The P19963 protein was observed to undergo intron retention at seedling stage (IR); a type of alternative splicing regulation events when intron is not spliced out after the action of splicing machinery. Experimental validation using RT-PCR and sanger sequencing also proved retention of intronic region in the mature mRNA transcript of P19963 protein. We observed that even if this gene was expressing at later stages of plant development i.e., pollen producing stage, but due to alternative splicing it became disrupted at the seedling stage. The non-functionality of protein can be beneficial or harmful but in our case as the gene is having IR and becomes non-functional, it is beneficial as the pollen allergies and skin allergies can be minimized at this stage or not required at all.</span></p></div>\",\"PeriodicalId\":38041,\"journal\":{\"name\":\"Plant Gene\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352407322000129\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352407322000129","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
摘要
选择性剪接调控(AS)是一种重要的转录后基因调控机制,可增加转录组和随后的蛋白质组的复杂性和多样性。本研究通过对木棉的转录组学分析,探讨花粉过敏原(Ole e 1) P19963蛋白的选择性剪接调控过程。P19963因其酸性而引起皮肤过敏。P19963蛋白在苗期出现内含子滞留(IR);当内含子在剪接机制的作用下没有被剪接出来时,就会发生一种选择性剪接调节。利用RT-PCR和sanger测序的实验验证也证实了P19963蛋白成熟mRNA转录物中保留内含子区域。我们观察到,即使该基因在植物发育的后期,即花粉产生期表达,但由于选择性剪接,它在幼苗期被破坏。蛋白质的非功能性可能是有益的,也可能是有害的,但在我们的案例中,由于基因具有IR并变得无功能,它是有益的,因为花粉过敏和皮肤过敏在这个阶段可以最小化,或者根本不需要。
Alternative splicing plays a vital role in regulating pollen allergen (Ole e 1) P19963 protein in Gossypium arboreum
Alternative splicing regulation (AS) is an important post-transcriptional gene regulatory mechanism for increasing the complexity and diversity of transcriptomes and subsequently proteomes. In this study, we performed transcriptomic analysis of Gossypium arboreum to explore the process of alternative splicing regulation in pollen allergen (Ole e 1) P19963 protein. The P19963 causes skin allergies because of its acidic properties. The P19963 protein was observed to undergo intron retention at seedling stage (IR); a type of alternative splicing regulation events when intron is not spliced out after the action of splicing machinery. Experimental validation using RT-PCR and sanger sequencing also proved retention of intronic region in the mature mRNA transcript of P19963 protein. We observed that even if this gene was expressing at later stages of plant development i.e., pollen producing stage, but due to alternative splicing it became disrupted at the seedling stage. The non-functionality of protein can be beneficial or harmful but in our case as the gene is having IR and becomes non-functional, it is beneficial as the pollen allergies and skin allergies can be minimized at this stage or not required at all.
Plant GeneAgricultural and Biological Sciences-Plant Science
CiteScore
4.50
自引率
0.00%
发文量
42
审稿时长
51 days
期刊介绍:
Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.