大翅果甲醇提取物精油组成及抗氧化、抗菌活性研究

IF 1.1 Q4 IMMUNOLOGY
Ali Valibeik, Narges Tavakoli, H. Amiri, Rouhollah Heydari, Leila Hasanvand, R. Rostami, Niloufar Tavakoli Dastjerd, H. Ahmadvand
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引用次数: 0

摘要

背景:桔梗是一种原产于伊朗的安科药用植物。目的:研究翅翅小蠊地上部位挥发油的植物化学成分及甲醇提取物的抑菌和抗氧化活性。材料与方法:采用气相色谱-质谱联用法(GC-MS)、DPPH(2,2-二苯基-1-苦味酰肼)测定法和微量稀释法测定其挥发油的主要成分,研究其抗氧化和抗菌活性。结果:气相色谱-质谱分析表明,花、果、叶中挥发油的主要成分分别为辛酸丁酯(24.88%)、9-十八烯酸(14.19%)和α -蒎烯(19.28%)。叶甲醇提取物中酚类化合物含量最高(0.5±0.11 mgGAEs/g)。黄酮类化合物含量最高(0.074±12.4 mg QEs/g)。DPPH实验显示,乙醇提取物对自由基的清除能力最强(IC50为201.7±20.39 μg/mL)。果实的总抗氧化能力最高(0.004±0.0005 g AAE/ml)。采用微量肉汤稀释法研究了植物提取物对病原菌和细菌的抑菌活性。最小杀菌浓度(MBC)为312 ~ 10000 mg/ml,最小抑菌浓度(MIC)为1000 ~ 10000 mg/ml。结论:本研究结果表明,该植物是一种有效的生物活性化合物来源,具有进一步研究的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Composition of the essential oils, antioxidant and antibacterial activities of the methanolic extract of Prangos uloptera
Background: Prangos uloptera is a medicinal plant from the Apiaceae family which is native to Iran. Objectives: In the current study, phytochemical constituents of the essential oils, antibacterial and antioxidant activities of methanolic extract of the aerial parts of P. uloptera were investigated. Materials and Methods: To determine the main ingredients of the essential oils and to investigate the antioxidant and antibacterial activities of this plant, the GC-MS (gas chromatography - mass spectrometry) analysis, DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and microdilution method were used. Results: The GC–MS analysis showed that the leading constituents of the essential oils were butyl octanoate (24.88%), 9-Octadecenoic acid (Z) (14.19%) and alpha-pinene (19.28%) in the flowers, fruit and leaves, respectively. The greatest amount of phenolic compound was observed in methanolic extract of the leaves (0.5 ± 0.11 mgGAEs/g). Likewise, the greatest level of flavonoids (0.074 ± 12.4 mg QEs/g) was identified in the leaves. Furthermore, DPPH assay showed the most radical scavenging activity (IC50 201.7 ± 20.39 μg/mL) in the methanolic extract of the leaves. The highest total antioxidant capacity was observed in the fruit (0.004 ± 0.0005 g AAE/ml). The antibacterial activity of the plant extracts against the pathogenic fungal and bacterial species was investigated by broth microdilution method. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) amounts were in the ranges of 312 to 10000 and 1000 to 10000 mg/ml, respectively. Conclusion: The results of this research demonstrated that P. uloptera was a potent source of useful bioactive compounds, making it a promising candidate for further studies.
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来源期刊
CiteScore
1.70
自引率
0.00%
发文量
65
审稿时长
3 weeks
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