Nbp35/ApbC同源物在产甲烷古菌中作为非必需的[4Fe‐4S]转移蛋白

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Cuiping Zhao, Zhe Lyu, Feng Long, Taiwo S Akinyemi, Kasidet Manakongtreecheep, D. Söll, W. Whitman, David J. Vinyard, Yuchen Liu
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引用次数: 3

摘要

核苷酸结合蛋白35 (Nbp35)/胞质Fe - S簇缺陷1 (Cfd1)/选择性嘧啶生物合成蛋白C (ApbC)蛋白同源物已在所有三个生命域中被鉴定出来。在真核生物中,Nbp35/Cfd1异复合体是细胞质和细胞核中Fe - S蛋白成熟所必需的铁- S簇组装支架,而细菌ApbC是一种铁- S簇转移蛋白,仅参与特定靶蛋白的成熟。在这里,我们发现从其原生古细菌宿主马里帕路甲烷球菌中纯化的Nbp35/ApbC同源物MMP0704含有一个可以转移到[4Fe‐4S]载子蛋白上的[4Fe‐4S]簇。在我们的测试条件下,mmp0704的缺失不会引起生长缺陷。我们的数据表明Nbp35/ApbC是产甲烷古菌中非必需的[4Fe‐4S]簇转移蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Nbp35/ApbC homolog acts as a nonessential [4Fe‐4S] transfer protein in methanogenic archaea
The nucleotide binding protein 35 (Nbp35)/cytosolic Fe‐S cluster deficient 1 (Cfd1)/alternative pyrimidine biosynthetic protein C (ApbC) protein homologs have been identified in all three domains of life. In eukaryotes, the Nbp35/Cfd1 heterocomplex is an essential Fe‐S cluster assembly scaffold required for the maturation of Fe‐S proteins in the cytosol and nucleus, whereas the bacterial ApbC is an Fe‐S cluster transfer protein only involved in the maturation of a specific target protein. Here, we show that the Nbp35/ApbC homolog MMP0704 purified from its native archaeal host Methanococcus maripaludis contains a [4Fe‐4S] cluster that can be transferred to a [4Fe‐4S] apoprotein. Deletion of mmp0704 from M. maripaludis does not cause growth deficiency under our tested conditions. Our data indicate that Nbp35/ApbC is a nonessential [4Fe‐4S] cluster transfer protein in methanogenic archaea.
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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
6.60
自引率
2.90%
发文量
303
审稿时长
1 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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