Abdallah M.A. Hassane , Taher M. Taha , Mohamed F. Awad , Hassan Mohamed , Mohammad Melebari
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Those isolates were characterized as <em>Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes</em> and <em>Fusarium chlamydosporum</em> according to morphological and phylogenetic bases<em>.</em> These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in <em>P. commune</em> (148.24 mg/g), followed by <em>F. chlamydosporum</em> (124.25 mg/g), while the highest total flavonoids were determined in <em>A. flavipes</em>, followed by <em>F. chlamydosporum, P. glaucoroseum, P. commune,</em> respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC<sub>50</sub> = 2.13 mg/mL exhibited by <em>P. commune</em>, followed by <em>F. chlamydosporum</em> (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC.</p></div><div><h3>Conclusions</h3><p>Endophytic fungi derived from medicinal plants are a promising candidate for the wide industrial production of antioxidant agents with several useful medical and pharmaceutical applications.</p><p><strong>How to cite:</strong> Hassane A. M. A, Taha T. M, Awad M. F, et al. Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 37-45"},"PeriodicalIF":2.3000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000185/pdfft?md5=4f851b7954ea67ddfa97583a1c086101&pid=1-s2.0-S0717345822000185-main.pdf","citationCount":"9","resultStr":"{\"title\":\"Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia\",\"authors\":\"Abdallah M.A. Hassane , Taher M. Taha , Mohamed F. Awad , Hassan Mohamed , Mohammad Melebari\",\"doi\":\"10.1016/j.ejbt.2022.05.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Endophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aimed to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity.</p></div><div><h3>Results</h3><p>In the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants <em>Rumex nervosus</em>, <em>Pulicaria crispa</em> and <em>Withania somnifera</em>. Those isolates were characterized as <em>Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes</em> and <em>Fusarium chlamydosporum</em> according to morphological and phylogenetic bases<em>.</em> These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in <em>P. commune</em> (148.24 mg/g), followed by <em>F. chlamydosporum</em> (124.25 mg/g), while the highest total flavonoids were determined in <em>A. flavipes</em>, followed by <em>F. chlamydosporum, P. glaucoroseum, P. commune,</em> respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC<sub>50</sub> = 2.13 mg/mL exhibited by <em>P. commune</em>, followed by <em>F. chlamydosporum</em> (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. 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引用次数: 9
摘要
内生真菌存在于植物组织中，对人体无害。它们产生多种代谢物，是生物活性物质的丰富来源。本研究旨在鉴定不同药用植物中分离的真菌，评价其抗氧化活性，并检测其抗氧化活性成分。结果从不同药用植物中分离得到4株内生真菌。根据形态和系统发育特征，分离菌株分别为青霉菌、青霉菌、黄曲霉和衣孢镰刀菌。将这些菌株在腐殖水稻上繁殖，测定其乙酸乙酯粗提物的总酚、总黄酮和抗DPPH自由基的能力。结果表明，黄芪中酚类成分含量最高，分别为黄芪(148.24 mg/g)、黄芪(124.25 mg/g)、黄芪(148.24 mg/g)和黄芪(124.25 mg/g)。对羟基自由基的清除能力最强的菌群为青松菌(P. commune)， IC50值为2.13 mg/mL，其次为衣孢菌(F. chlamydosporum)， IC50值为2.68 mg/mL。所研究的菌株对致病性分离株和肝癌细胞株(HepG2)有较弱的抑菌和细胞毒作用。通过HPLC检测不同酚类、黄酮类和酚酸的含量，对真菌乙酸乙酯提取物进行了定量表征。结论药用植物内生真菌具有广泛的工业生产抗氧化剂的潜力，具有多种药用价值。引用方式:Hassane A. M. A, Taha T. M, Awad M. F等。沙特阿拉伯药用植物内生真菌的自由基清除能力、高效液相色谱分析和系统发育分析。中国生物医学工程学报(英文版);2009;16。https://doi.org/10.1016/j.ejbt.2022.05.001
Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia
Background
Endophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aimed to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity.
Results
In the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants Rumex nervosus, Pulicaria crispa and Withania somnifera. Those isolates were characterized as Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes and Fusarium chlamydosporum according to morphological and phylogenetic bases. These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in P. commune (148.24 mg/g), followed by F. chlamydosporum (124.25 mg/g), while the highest total flavonoids were determined in A. flavipes, followed by F. chlamydosporum, P. glaucoroseum, P. commune, respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC50 = 2.13 mg/mL exhibited by P. commune, followed by F. chlamydosporum (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC.
Conclusions
Endophytic fungi derived from medicinal plants are a promising candidate for the wide industrial production of antioxidant agents with several useful medical and pharmaceutical applications.
How to cite: Hassane A. M. A, Taha T. M, Awad M. F, et al. Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.001
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
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• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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