一种新的Dietzia菌株的好氧和厌氧还原硼铁矿

IF 0.9 4区 地球科学 Q4 GEOCHEMISTRY & GEOPHYSICS
Huiqin Zhang, Yan Li, Xin Wang, Anhuai Lu, Hongrui Ding, Cuiping Zeng, Xiao Wang, Xiaolei Wu, Yong Nie, Changqiu Wang
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引用次数: 13

摘要

锰氧化物存在于各种地质环境中,对沉积物和土壤的成分和化学行为产生相当大的影响。锰氧化物的微生物还原是一个重要的过程,存在于许多不同的环境中,包括海洋和淡水沉积物、湖泊、缺氧盆地以及海洋的缺氧过渡带。虽然两种模式细菌(Geobacter和Shewanella)厌氧还原Mn的途径已经被深入研究,但Mn生物还原仍然是自然界中探索最少的过程。特别是,在O2存在的情况下,其他细菌对锰氧化物的还原在最近的出版物中很少有报道。通过一系列实验了解Dietzia DQ12-45-1b在生物还原铌矿中的作用。在厌氧系统中,4℃内Mn的还原率高达93%。接种1.0?×?1010个细胞/mL Dietzia DQ12-45-1b菌株。AQDS的加入使Mn的还原率从53%提高到91%。Mn的厌氧还原不随细菌蛋白质浓度的增加而增加,并且在2-14天的稳定阶段,Mn的还原速率与蛋白质浓度成正比。通过XRD、SEM和XANES等分析表明,硼镁矿的厌氧还原过程将Mn(II)释放到介质中或吸附在矿物或细菌表面,导致硼镁矿溶解。在好氧条件下,当细胞浓度为1.0 × ×时,还原率仅为37%。1010个细胞/mL,远低于平行厌氧处理。好氧条件下细菌的生长表现为蛋白质和ph随时间的增加。与厌氧实验相比,添加AQDS使Mn的还原率从25%降低到6%。还原的Mn(II)与乙酸代谢产生的二氧化碳结合,以及细胞生长所给予的碱性环境,最终形成了含Mn(II)的碳酸盐(kutnohorite), XRD和XANES结果证实了这一点。细胞浓度最高的体系为1.0 ×?1010个细胞/mL产生的库氏体最多,而细胞浓度为6.2 × × ×时产生的Mn(II)浓度最高。108个细胞/mL的浓度太低,在热力学上不利于库氏石的形成,反而导致文石的形成。Dietzia DQ12-45-1b能够厌氧和好氧还原伯尼锡。Mn(IV)的还原速率和程度取决于细胞浓度、是否添加AQDS、是否存在O2。同时,Mn(IV)的生物还原程度和悬浮条件决定了不溶性矿物产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Aerobic and anaerobic reduction of birnessite by a novel Dietzia strain

Aerobic and anaerobic reduction of birnessite by a novel Dietzia strain

Mn oxides occur in a wide variety of geological settings and exert considerable influences on the components and chemical behaviors of sediments and soils. Microbial reduction of Mn oxides is an important process found in many different environments including marine and freshwater sediments, lakes, anoxic basins, as well as oxic-anoxic transition zone of ocean. Although the pathway of Mn anaerobic reduction by two model bacteria, Geobacter and Shewanella, has been intensively studied, Mn bio-reduction is still the least well-explored process in nature. Particularly, reduction of Mn oxides by other bacteria and in the presence of O2 has been fewly reported in recent publishes.

A series of experiments were conducted to understand the capability of Dietzia DQ12-45-1b in bioreduction of birnessite. In anaerobic systems, Mn reduction rate reached as high as 93% within 4?weeks when inoculated with 1.0?×?1010 cells/mL Dietzia DQ12-45-1b strains. Addition of AQDS enhanced Mn reduction rate from 53 to 91%. The anaerobic reduction of Mn was not coupled by any increase in bacterial protein concentration, and the reduction rate in the stable stage of day 2–14 was found to be in good proportion to the protein concentration. The anaerobic reduction of birnessite released Mn(II) either into the medium or adsorbed on the mineral or bacteria surface and resulted in the dissolution of birnessite as indicated by XRD, SEM and XANES. Under aerobic condition, the reduction rate was only 37% with a cell concentration of 1.0?×?1010 cells/mL, much lower than that in parallel anaerobic treatment. Bacterial growth under aerobic condition was indicated by time-course increase of protein and pH. In contrast to anaerobic experiments, addition of AQDS decreased Mn reduction rate from 25 to 6%. The reduced Mn(II) combined with carbon dioxide produced by acetate metabolism, as well as an alkaline pH environment given by cell growth, finally resulted in the formation of Mn(II)-bearing carbonate (kutnohorite), which was verified by XRD and XANES results. The system with the highest cell concentration of 1.0?×?1010 cells/mL gave rise to the most amount of kutnohorite, while concentration of Mn(II) produced with cell concentration of 6.2?×?108 cells/mL was too low to thermodynamically favor the formation of kutnohorite but result in the formation of aragonite instead.

Dietzia DQ12-45-1b was able to anaerobically and aerobically reduce birnessite. The rate and extent of Mn(IV) reduction depend on cell concentration, addition of AQDS or not, and presence of O2 or not. Meanwhile, Mn(IV) bioreduction extent and suspension conditions determined the insoluble mineral products.

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来源期刊
Geochemical Transactions
Geochemical Transactions 地学-地球化学与地球物理
CiteScore
3.70
自引率
4.30%
发文量
2
审稿时长
>12 weeks
期刊介绍: Geochemical Transactions publishes high-quality research in all areas of chemistry as it relates to materials and processes occurring in terrestrial and extraterrestrial systems.
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