Iacopo Ruolo , Sara Napolitano , Davide Salzano , Mario di Bernardo , Diego di Bernardo
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Control engineering meets synthetic biology: Foundations and applications
Synthetic Biology enables the construction of new genetic circuits with the final goal of controlling cellular behaviour. However, the noisy nature of biomolecular interactions renders a fine regulation of such circuits necessary for their correct operation. A possible solution is cybergenetics, a new discipline merging the tools of Synthetic biology with those of control theory. Biomolecular controllers can be classified into three different categories (i) embedded, in which the controller is implemented as a genetic circuit co-existing in the same cell with the process to be controlled; (ii) external, where the controller is implemented as a software in a computer; (iii) multicellular, in which the controller and the process to be controlled are in two different cell populations. Here, we describe the advantages and drawbacks of each one of the approaches, expounding their main advantages, limitations, and applications.
期刊介绍:
Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution