蜂蜜与纳米银对尿路感染病原菌的协同作用

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
N. Guruvu, K. Gayathri, D. Lakshmi Lalitha, L. S. Simhachalam Kutikuppala, P. Vegi
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引用次数: 1

摘要

尿路感染(uti)是最普遍的细菌感染,每年影响全球1.5亿人。尿路感染可由多种病原体引起,但大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和神奇变形杆菌是最常见的罪魁祸首。耐抗生素细菌的生长和发展是目前国际卫生界的一个主要担忧。寻找一种替代疗法可以有效地解决这个问题。蜂蜜的pH值较低(平均4.4),不利于细菌生长;未稀释的蜂蜜与银纳米颗粒(AgNPs)结合显示出协同抗菌作用,有助于减少感染。目的研究含AgNPs蜂蜜对尿分离病原菌的抑菌作用。患者与方法采用目的采样技术,对30例尿路感染患者进行尿液采集。采用半定量方法在麦康基琼脂和血琼脂上培养尿液标本。孵育24小时后,培养皿上可见细菌生长。然后,计数菌落,用cfu数乘以1000计算每毫升原始材料中微生物的数量。结果30份尿样中共分离出10株细菌,经生化鉴定为3种不同的菌株。最常见的大肠杆菌菌株占82%,其次是铜绿假单胞菌(12%)和神奇假单胞菌(6%)。在用未稀释蜂蜜与AgNPs混合检测的所有三种生物体中,与仅蜂蜜、AgNPs和稀释蜂蜜与AgNPs混合相比,抑制区更为明显。结论多花蜂蜜联合AgNPs对泌尿系感染有较好的治疗作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic effect of honey in combination with silver nanoparticles on isolated pathogens from urinary tract infection
Background Urinary tract infections (UTIs) are the most prevalent bacterial infections, affecting 150 million people worldwide each year. UTIs can be caused by a variety of pathogens, but Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Proteus mirabilis are the most frequent culprits. The growth and development of antibiotic-resistant bacteria are currently a major worry for the international health community. Looking for a treatment substitute could be effective in tackling this issue. The honey has a low pH (mean 4.4) value, which makes it unfavorable for bacterial growth; undiluted honey combination with silver nanoparticles (AgNPs) shows synergistic antimicrobial effect and helps lessen infection. Aim This study was undertaken to find out the effectiveness of honey containing AgNPs in the antibacterial activity of isolated urine pathogens. Patients and methods A total of 30 patients with UTI were included through purposive sampling technique, and urine samples were collected from them. A semiquantitative approach was used to cultivate specimens of urine on MacConkey agar and blood agar. After 24 h of incubation, bacterial growth was seen on the plates. Then, colonies were counted and the number of microorganisms per milliliter in the original material was calculated by multiplying the number of CFUs by 1000. Results A total of 10 bacterial isolates were found in the 30 urine samples, and biochemical studies showed that these isolates were from three different species. The most common strain of E. coli represented 82%, followed by P. aeruginosa (12%) and P. mirabilis (6%). In all three organisms examined with undiluted honey mixed with AgNPs, the zone of inhibition was more pronounced when compared with only honey, AgNPs, and diluted honey mixed with AgNPs. Conclusion The results of the current investigation showed that multifloral honey combined with AgNPs was effective against urinary infections.
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
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0.00%
发文量
37
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