倍半萜化合物α-密码酮减轻氧-葡萄糖剥夺/复氧神经元的损伤

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2022-06-06 DOI:10.3329/bjp.v17i2.58188

Jian Wang, Xuefeng Gao

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引用次数: 0

摘要

本实验旨在探讨香附提取物α-cyperone对PC12细胞的作用及其机制。氧-糖剥夺/再氧化(OGD/R)诱导细胞后，观察细胞活力、形态学、炎症、氧化应激和凋亡水平。为了探讨α-cyperone的作用机制，我们用sirtuin-3 (SIRT3)抑制剂3-TYP处理细胞，然后评估3-TYP对α-cyperone功能的影响。α-赛柏酮可降低OGD/ r诱导的神经元活力损伤，减轻炎症、氧化应激和细胞凋亡。此外，α-cyperone可以提高SIRT3水平，降低乙酰叉头盒O1 (FOXO1)水平，而3-TYP则打破了α-cyperone在PC12细胞中上述方面的作用。综上所述，α-cyperone可激活SIRT3和FOXO1去乙酰化，减轻OGD/ r诱导的细胞炎症、氧化应激和凋亡。

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Sesquiterpene compound α-cyperone relieves the injury in neurons undergoing oxygen-glucose deprivation/reoxygenation

The present study aimed to explore the effects of α-cyperone, an extract of Cyperus rotundus, on PC12 cells, as well as the underlying mechanism. Following the cells were induced by oxygen-glucose deprivation/reoxygena-tion (OGD/R), the viability, morphology, inflammation, oxidative stress and apoptotic levels in the cells were evaluated. To explore the mechanism of α-cyperone, cells were treated with 3-TYP, a sirtuin-3 (SIRT3) inhibitor, and then the effects of 3-TYP on the function of α-cyperone were assessed. α-Cyperone was found to reduce OGD/R-induced damage to neuronal viability and alleviate inflammation, oxidative stress, and apoptosis. In addition, α-cyperone could elevate SIRT3 and decline acetyl-forkhead box O1 (FOXO1) levels, and 3-TYP broke the effects of α-cyperone on the aforementioned aspects in the PC12 cells. In conclusion, α-cyperone activated SIRT3 and FOXO1 deacetylation, and alleviated OGD/R-induced cell inflammation, oxidative stress, and apoptosis.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.