用无标记蛋白质组学方法对山羊肉进行分析表明，MYL3是一种潜在的肉韧性分子标记

IF 1.4 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Czech Journal of Animal Science Pub Date : 2022-04-30 DOI:10.17221/61/2021-cjas

Yuchun Xie, Nai Rile, Xuewu Li, Haijun Li, Meng Zhao, Tianyu Che, Ting Cai, Zhi-hong Liu, Jinquan Li

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引用次数: 4

摘要

内蒙古绒山羊以其明亮的白色羊绒纤维而闻名。然而，人们很少注意到这个品种的肉的优良特性。我们采用无标记蛋白质组学方法分析了五种不同肌肉中的总蛋白质含量，共检测到1227种蛋白质。通过连续窗口获取所有理论片段离子（SWATH），在五块肌肉中成功检测到16、33、49、39和31种差异表达的蛋白质。差异表达蛋白质的蛋白质-蛋白质相互作用网络分析揭示了许多与脂肪酸β氧化和肌肉发育有关的强烈相互作用。基于5块肌肉中的SWATH，检测到25种与肌肉发育相关的差异表达蛋白，包括7种肌纤维结构蛋白（ACTG2、ACTN4、TAGLN、MYL3、MYL1、MYL6B和MYH4）。最后，对MYL3的免疫组织化学分析表明，MYL3的比例可能是肌肉韧性的潜在分子标记。

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Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness

The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed’s meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein–protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.

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来源期刊

Czech Journal of Animal Science Agriculture, Dairy & Animal Science-奶制品与动物科学

CiteScore

2.40

自引率

16.70%

发文量

审稿时长

5 months

期刊介绍： Original scientific papers and critical reviews covering all areas of genetics and breeding, physiology, reproduction, nutrition and feeds, technology, ethology and economics of cattle, pig, sheep, goat, poultry, fish and other farm animal management. Papers are published in English.