使用聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）纳米颗粒向骨肉瘤细胞靶向递送依托泊苷

IF 1.1 4区生物学 Q3 BIOLOGY

Turkish Journal of Biology Pub Date : 2017-10-01 DOI:10.3906/BIY-1612-17

E. Alp, T. Çırak, M. Demirbilek, M. Türk, Eylem Güven

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引用次数: 7

摘要

制备了叶酸(FA)功能化聚(3-羟基丁酸-co-3-羟基戊酸)(PHBV)纳米颗粒，以提高抗癌药物依托泊苷的递送效率，用于骨肉瘤的临床治疗。采用乳化/溶剂蒸发法制备了PHBV纳米颗粒，粒径范围为200 ~ 250 nm, zeta电位范围为-21和-27 mV。考察其包封率和体外释药效果。我们还利用Saos-2骨肉瘤细胞研究了PHBV纳米颗粒的细胞毒性、凋亡和坏死作用。本研究结果表明，依托泊苷负载和fa功能化的PHBV纳米颗粒可以成功用于骨肉瘤的靶向治疗。

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Targeted delivery of etoposide to osteosarcoma cells using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles

Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposide-loaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.

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来源期刊

Turkish Journal of Biology BIOLOGY-

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.