印迹X染色体失活影响小鼠囊胚原始内胚层分化

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
A. Fukuda, N. Motosugi, Mikiko Ando, M. Kimura, A. Umezawa, H. Akutsu
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引用次数: 0

摘要

表观遗传学和转录组的改变对谱系规范至关重要,表现为雌性小鼠植入前胚胎中的印迹X染色体失活(iXCI)。然而,各种因素如何影响转录组状态和谱系承诺仍不清楚。我们发现,与iXCI损失相比,体外培养持续时间强烈影响转录变异。对主要转录和表观基因组因子的内部细胞团(ICM)的单细胞分析显示,原始内胚层(PrE)中iXCI的缺失减少了性别特异性表达差异,但在外胚层中没有。与雄性相比,雌性的ICM比例更高,并且PrE的发育受到雌性胚胎中iXCI状态的影响。我们的发现为性别差异和iXCI在谱系规范中的功能提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Imprinted X‐chromosome inactivation impacts primitive endoderm differentiation in mouse blastocysts
Epigenetic and transcriptome alterations are essential for lineage specification, represented by imprinted X‐chromosome inactivation (iXCI) in female mouse preimplantation embryos. However, how various factors affect transcriptome states and lineage commitment remains unclear. We found that in vitro culture duration strongly influences transcriptional variation compared to iXCI loss. Single‐cell analysis of the inner cell mass (ICM) for major transcription and epigenomic factors revealed that sex‐specific differences in expression are diminished by loss of iXCI in the primitive endoderm (PrE) but not in the epiblast. Females had a higher proportion of ICM compared to that in males, and PrE development was affected by iXCI states in female embryos. Our findings provide insight into sex differences and iXCI function in lineage specification.
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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
6.60
自引率
2.90%
发文量
303
审稿时长
1 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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