Gisele Amaro Teixeira, Riudo de Paiva Ferreira, Denilce Meneses Lopes
{"title":"比较细胞遗传学分析揭示了五种无刺蜂属的染色体变异(蜂科,蜂科,蜂科)","authors":"Gisele Amaro Teixeira, Riudo de Paiva Ferreira, Denilce Meneses Lopes","doi":"10.1007/s13592-023-01002-2","DOIUrl":null,"url":null,"abstract":"<div><p>The stingless bee genus <i>Trigona</i> includes 32 species, exclusive to the New World, which are grouped into two clades (A and B) according to phylogenetic molecular data. Cytogenetic studies have been performed in only seven <i>Trigona</i> taxa, and molecular cytogenetic data are available for only one species. These studies have been important for the chromosomal characterization of the species; however, discussions focusing on the karyotype evolution of <i>Trigona</i> in a phylogenetic context are lacking. In this study, we characterized the karyotype, through classical and molecular cytogenetics, of five <i>Trigona</i> species: <i>T. pallens</i> and <i>T. williana</i>, from clade A, and <i>T. hypogea</i>, <i>T.</i> aff<i>. fuscipennis</i>, and <i>T. truculenta</i>, from clade B, in order to provide insights into the karyotype evolution in <i>Trigona</i> and investigate whether the analyzed cytogenetic markers may have a phylogenetic signal. All five <i>Trigona</i> species have 2n = 34 chromosomes. Variations in the karyotype formula were observed in <i>T. truculenta</i> and <i>T. hypogea</i> compared with the other three species. Although heterochromatin distribution was restricted to one of the arms in most of the chromosomes of the five species, C-banding experiments highlighted a lower degree of heterochromatin compaction in <i>T. pallens</i> and <i>T. williana</i>. The microsatellite (GA)<sub>15</sub> was exclusively located in the euchromatic regions of the chromosomes in all five species. The number of pairs bearing rDNA genes varied among the species studied, and this cytogenetic trait seems to reflect the phylogeny, separating the species into two clades. This study showed interspecific variations to a greater or lesser degree among <i>Trigona</i> species, highlighting the intense chromosomal evolutionary dynamics in the genus.\n</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-023-01002-2.pdf","citationCount":"1","resultStr":"{\"title\":\"Comparative cytogenetic analysis reveals chromosomal variability in five stingless bees of the genus Trigona (Apidae, Apinae, Meliponini)\",\"authors\":\"Gisele Amaro Teixeira, Riudo de Paiva Ferreira, Denilce Meneses Lopes\",\"doi\":\"10.1007/s13592-023-01002-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The stingless bee genus <i>Trigona</i> includes 32 species, exclusive to the New World, which are grouped into two clades (A and B) according to phylogenetic molecular data. Cytogenetic studies have been performed in only seven <i>Trigona</i> taxa, and molecular cytogenetic data are available for only one species. These studies have been important for the chromosomal characterization of the species; however, discussions focusing on the karyotype evolution of <i>Trigona</i> in a phylogenetic context are lacking. In this study, we characterized the karyotype, through classical and molecular cytogenetics, of five <i>Trigona</i> species: <i>T. pallens</i> and <i>T. williana</i>, from clade A, and <i>T. hypogea</i>, <i>T.</i> aff<i>. fuscipennis</i>, and <i>T. truculenta</i>, from clade B, in order to provide insights into the karyotype evolution in <i>Trigona</i> and investigate whether the analyzed cytogenetic markers may have a phylogenetic signal. All five <i>Trigona</i> species have 2n = 34 chromosomes. Variations in the karyotype formula were observed in <i>T. truculenta</i> and <i>T. hypogea</i> compared with the other three species. Although heterochromatin distribution was restricted to one of the arms in most of the chromosomes of the five species, C-banding experiments highlighted a lower degree of heterochromatin compaction in <i>T. pallens</i> and <i>T. williana</i>. The microsatellite (GA)<sub>15</sub> was exclusively located in the euchromatic regions of the chromosomes in all five species. The number of pairs bearing rDNA genes varied among the species studied, and this cytogenetic trait seems to reflect the phylogeny, separating the species into two clades. This study showed interspecific variations to a greater or lesser degree among <i>Trigona</i> species, highlighting the intense chromosomal evolutionary dynamics in the genus.\\n</p></div>\",\"PeriodicalId\":8078,\"journal\":{\"name\":\"Apidologie\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s13592-023-01002-2.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Apidologie\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13592-023-01002-2\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Apidologie","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13592-023-01002-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Comparative cytogenetic analysis reveals chromosomal variability in five stingless bees of the genus Trigona (Apidae, Apinae, Meliponini)
The stingless bee genus Trigona includes 32 species, exclusive to the New World, which are grouped into two clades (A and B) according to phylogenetic molecular data. Cytogenetic studies have been performed in only seven Trigona taxa, and molecular cytogenetic data are available for only one species. These studies have been important for the chromosomal characterization of the species; however, discussions focusing on the karyotype evolution of Trigona in a phylogenetic context are lacking. In this study, we characterized the karyotype, through classical and molecular cytogenetics, of five Trigona species: T. pallens and T. williana, from clade A, and T. hypogea, T. aff. fuscipennis, and T. truculenta, from clade B, in order to provide insights into the karyotype evolution in Trigona and investigate whether the analyzed cytogenetic markers may have a phylogenetic signal. All five Trigona species have 2n = 34 chromosomes. Variations in the karyotype formula were observed in T. truculenta and T. hypogea compared with the other three species. Although heterochromatin distribution was restricted to one of the arms in most of the chromosomes of the five species, C-banding experiments highlighted a lower degree of heterochromatin compaction in T. pallens and T. williana. The microsatellite (GA)15 was exclusively located in the euchromatic regions of the chromosomes in all five species. The number of pairs bearing rDNA genes varied among the species studied, and this cytogenetic trait seems to reflect the phylogeny, separating the species into two clades. This study showed interspecific variations to a greater or lesser degree among Trigona species, highlighting the intense chromosomal evolutionary dynamics in the genus.
期刊介绍:
Apidologie is a peer-reviewed journal devoted to the biology of insects belonging to the superfamily Apoidea.
Its range of coverage includes behavior, ecology, pollination, genetics, physiology, systematics, toxicology and pathology. Also accepted are papers on the rearing, exploitation and practical use of Apoidea and their products, as far as they make a clear contribution to the understanding of bee biology.
Apidologie is an official publication of the Institut National de la Recherche Agronomique (INRA) and Deutscher Imkerbund E.V. (D.I.B.)