以未修饰金纳米颗粒为比色探针的无标记适配体为基础的水介质中汞离子的比色检测

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Li Li, Baoxin Li, Yingying Qi, Yan Jin
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引用次数: 209

摘要

我们报道了一种简单、灵敏的基于适配体的汞离子(Hg2+)比色检测方法,使用未修饰的金纳米颗粒作为比色探针。这是基于这样一个事实,即裸金纳米颗粒与短单链DNA和双链DNA的相互作用不同。抗Hg2+适配体富含胸腺嘧啶(T),在Hg2+存在下容易形成T -Hg2+ -T构型。通过测量颜色变化或吸附比,裸金纳米粒子可以有效区分Hg2+诱导的适体在给定高浓度盐存在下的构象变化。实验表明,在50年的1 × 10 × 4°范围内,Hg2+浓度呈线性响应。摩尔L ?1到1 × 10 × 9?摩尔L ? 1。即使用肉眼,我们也可以在几分钟内识别出微摩尔的Hg2+浓度。采用分光光度法,将检测限提高到纳摩尔范围(0.6?nM)。该方法对Hg2+的选择性优于K+、Ba2+、Ni2+、Pb2+、Cu2+、Cd2+、Mg2+、Ca2+、Zn2+、Al3+和Fe3+等金属阳离子。这种Hg2+测定法的主要优点是其水溶性,简单,低成本,视觉比色法和高灵敏度。该方法为Hg2+的检测提供了一种潜在的有用工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Label-free aptamer-based colorimetric detection of mercury ions in aqueous media using unmodified gold nanoparticles as colorimetric probe

Label-free aptamer-based colorimetric detection of mercury ions in aqueous media using unmodified gold nanoparticles as colorimetric probe

We report a simple and sensitive aptamer-based colorimetric detection of mercury ions (Hg2+) using unmodified gold nanoparticles as colorimetric probe. It is based on the fact that bare gold nanoparticles interact differently with short single-strand DNA and double-stranded DNA. The anti-Hg2+ aptamer is rich in thymine (T) and readily forms T–Hg2+–T configuration in the presence of Hg2+. By measuring color change or adsorption ratio, the bare gold nanoparticles can effectively differentiate the Hg2+-induced conformational change of the aptamer in the presence of a given salt with high concentration. The assay shows a linear response toward Hg2+ concentration through a five-decade range of 1?×?10?4?mol L?1 to 1?×?10?9?mol L?1. Even with the naked eye, we could identify micromolar Hg2+ concentrations within minutes. By using the spectrometric method, the detection limit was improved to the nanomolar range (0.6?nM). The assay shows excellent selectivity for Hg2+ over other metal cations including K+, Ba2+, Ni2+, Pb2+, Cu2+, Cd2+, Mg2+, Ca2+, Zn2+, Al3+, and Fe3+. The major advantages of this Hg2+ assay are its water-solubility, simplicity, low cost, visual colorimetry, and high sensitivity. This method provides a potentially useful tool for the Hg2+ detection.

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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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