Aydin Tahmasebifar , Erkan Türker Baran , Bengi Yilmaz , Ahmet Engin Pazarceviren
{"title":"用于骨组织工程的生物玻璃-海藻酸盐/羧甲基纤维素生物打印","authors":"Aydin Tahmasebifar , Erkan Türker Baran , Bengi Yilmaz , Ahmet Engin Pazarceviren","doi":"10.1016/j.bprint.2023.e00296","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span><span>Bone regenerative medicine requires suitable substitutes that promote osteogenesis. Most of the bio-macromolecular hydrogels are promising because they are biocompatible and biodegradable, but their viscoelastic properties make them challenging to use, especially in </span>3D bioprinting applications. This study aimed to enhance the </span>mechanical properties of a bone substitute made of bioprinted </span>alginate<span>, carboxymethyl cellulose<span>, and 58S bioglass. We used dual cross-linking and optimized the concentration of cross-linking agents to improve hydrogel biological activity and mechanical stability. The compression test indicated that the combination of Ca</span></span></span><sup>2+</sup> and Fe<sup>3+</sup> significantly improved the mechanical properties of the alginate/carboxymethyl cellulose hydrogel. The hydrogel crosslinked with 4% Ca<sup>2+</sup> and 1.5% Fe<sup>3+</sup><span> showed the highest Young's modulus<span>. The study also found that the hydrogel rigidity influenced cell proliferation<span> capability during bioprinting, as observed in the cell viability results. At day 7, the cell viability of the bioprinted constructs cross-linked with 0.5% and 1% Fe</span></span></span><sup>3+</sup><span> exhibited significant increases. Similarly, these groups also demonstrated the highest alkaline phosphatase (ALP) activity at the same time. Results suggested that cross-linking density and resultant rigidity achieved by optimal concentrations of Fe</span><sup>3+</sup> have very significant effects on cell viability and osteogenesis.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioprinting of bioglass-alginate/carboxymethyl cellulose for bone tissue engineering\",\"authors\":\"Aydin Tahmasebifar , Erkan Türker Baran , Bengi Yilmaz , Ahmet Engin Pazarceviren\",\"doi\":\"10.1016/j.bprint.2023.e00296\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span><span>Bone regenerative medicine requires suitable substitutes that promote osteogenesis. Most of the bio-macromolecular hydrogels are promising because they are biocompatible and biodegradable, but their viscoelastic properties make them challenging to use, especially in </span>3D bioprinting applications. This study aimed to enhance the </span>mechanical properties of a bone substitute made of bioprinted </span>alginate<span>, carboxymethyl cellulose<span>, and 58S bioglass. We used dual cross-linking and optimized the concentration of cross-linking agents to improve hydrogel biological activity and mechanical stability. The compression test indicated that the combination of Ca</span></span></span><sup>2+</sup> and Fe<sup>3+</sup> significantly improved the mechanical properties of the alginate/carboxymethyl cellulose hydrogel. The hydrogel crosslinked with 4% Ca<sup>2+</sup> and 1.5% Fe<sup>3+</sup><span> showed the highest Young's modulus<span>. The study also found that the hydrogel rigidity influenced cell proliferation<span> capability during bioprinting, as observed in the cell viability results. At day 7, the cell viability of the bioprinted constructs cross-linked with 0.5% and 1% Fe</span></span></span><sup>3+</sup><span> exhibited significant increases. Similarly, these groups also demonstrated the highest alkaline phosphatase (ALP) activity at the same time. Results suggested that cross-linking density and resultant rigidity achieved by optimal concentrations of Fe</span><sup>3+</sup> have very significant effects on cell viability and osteogenesis.</p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886623000398\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000398","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
Bioprinting of bioglass-alginate/carboxymethyl cellulose for bone tissue engineering
Bone regenerative medicine requires suitable substitutes that promote osteogenesis. Most of the bio-macromolecular hydrogels are promising because they are biocompatible and biodegradable, but their viscoelastic properties make them challenging to use, especially in 3D bioprinting applications. This study aimed to enhance the mechanical properties of a bone substitute made of bioprinted alginate, carboxymethyl cellulose, and 58S bioglass. We used dual cross-linking and optimized the concentration of cross-linking agents to improve hydrogel biological activity and mechanical stability. The compression test indicated that the combination of Ca2+ and Fe3+ significantly improved the mechanical properties of the alginate/carboxymethyl cellulose hydrogel. The hydrogel crosslinked with 4% Ca2+ and 1.5% Fe3+ showed the highest Young's modulus. The study also found that the hydrogel rigidity influenced cell proliferation capability during bioprinting, as observed in the cell viability results. At day 7, the cell viability of the bioprinted constructs cross-linked with 0.5% and 1% Fe3+ exhibited significant increases. Similarly, these groups also demonstrated the highest alkaline phosphatase (ALP) activity at the same time. Results suggested that cross-linking density and resultant rigidity achieved by optimal concentrations of Fe3+ have very significant effects on cell viability and osteogenesis.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.