聚簇蛋白在预测妊娠早期和晚期子痫前期发展中的作用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
A. Timofeeva, IS Fedorov, A. Tarasova, KA Gorina, YuV Suhova, VA Gusar, TYu Ivanets
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引用次数: 1

摘要

先兆子痫(PE)发生在2-8%的妊娠中。它是孕产妇和围产期发病率和死亡率的主要原因之一。如今,从业者还没有采用任何测试来准确预测怀孕11至14周时PE的早期(第20至34周)或晚期(第34周后)发作。本研究旨在评估在妊娠早期使用分泌簇蛋白定量预测PE早期或晚期的可行性。该蛋白的选择一方面取决于其对细胞滋养层、合胞滋养层和细胞外滋养层细胞表达的特异性,另一方面,也取决于已证实的clusterin对滋养细胞的侵袭特性和子宫血管的妊娠转化的负面影响,而这些在PE的发病机制中起着关键作用。这项研究包括40名年龄在27-40岁之间的孕妇,她们在妊娠早期接受了全面的筛查。Western印迹显示,与生理性妊娠相比,PE情况下孕妇血清中分泌簇蛋白(40kDa)的水平显著增加:在早发性PE中,血清囊泡和囊外部分的簇蛋白水平增加了两倍(分别为p=0.03和p=0.004),迟发性PE——仅血清囊外部分增加三倍(p=0.002)。根据逻辑回归模型,妊娠早期孕妇血清囊外部分中分泌簇蛋白的水平在评估发生早发性PE(AUC=0.97,Se=1,Sp=0.875,临界值=0.3877)和晚发性PE的可能性方面具有预后意义(AUC=1,Se=1、Sp=1,临界值=0.5)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of clusterin in predicting development of early- and late-onset preeclampsia in the first trimester of pregnancy
Preeclampsia (PE) occurs in 2–8% of pregnancies. It is one of the leading causes of maternal and perinatal morbidity and mortality. Today, there are no tests adopted by the practitioners that enable accurate prediction of early (weeks 20 through 34) or late (after week 34) onset of PE when the pregnancy is in its 11th to 14th week. This study aimed to evaluate the feasibility of using secretory clusterin quantification to predict early or late PE during the first trimester of pregnancy. The choice of this protein is determined, on the one hand, by the specificity of its expression for cytotrophoblast, syncytiotrophoblast, and extracellular trophoblast cells, and, on the other hand, by the proven negative effect of clusterin on the invasive properties of trophoblastic cells and gestational transformations of uterine vessels, which play a key role in the pathogenesis of PE. The study included 40 pregnant women aged 27–40 years who underwent a comprehensive screening examination in the first trimester of pregnancy. Western blotting revealed a significant increase in the level of secretory clusterin (40 kDa) in the blood serum of pregnant women in the case of PE compared to physiological pregnancy: in early-onset PE, a twofold increase in the level of clusterin in the vesicular and extravesicular fractions of blood serum (p = 0.03 and p = 0.004, respectively), with late-onset PE — a threefold increase only in the extravesicular fraction of blood serum (p = 0.002). According to logistic regression models, the level of secretory clusterin in the extravesicular fraction of blood serum of pregnant women in the first trimester has prognostic significance in assessing the likelihood of developing early-onset PE (AUC = 0.97, Se = 1, Sp = 0.875, cutoff = 0.3877) and late-onset PE ( AUC = 1, Se = 1, Sp = 1, cutoff = 0.5).
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来源期刊
Bulletin of Russian State Medical University
Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-
CiteScore
0.80
自引率
0.00%
发文量
59
期刊介绍: Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.
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