荷斯坦奶牛和瑞士棕色奶牛MASP2、TG5和DQA1基因的遗传多态性

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-01-25 DOI:10.12681/jhvms.29524

A. El Zeer, M. Fouda, R. Darwish, A. Ateya

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引用次数: 0

摘要

本研究的目的是使用PCR-DNA测序技术在70头荷斯坦奶牛和棕色瑞士奶牛（每头35头）中研究MASP2、TG5和DQA1基因的多态性。在完全无菌条件下，将每只动物的血样收集到含有EDTA二钠作为抗凝剂的试管中，用于DNA提取。PCR扩增了305bp的MASP2、545bp的TG5和373bp的DQA1基因。DNA测序评估详细阐述了两个品种之间单核苷酸多态性（SNPs）形式的核苷酸序列变异。在荷斯坦品种的研究基因中鉴定出的SNPs为MASP2基因中的A46G和TG5基因中的C371T。在DQA1基因中，还报道了棕色瑞士品种特有的T53C SNP。卡方检验显示，所有已鉴定的SNPs的分布都存在显著差异（P＜0.001）。因此，已鉴定的单核苷酸多态性可作为荷斯坦和布朗-瑞士品种有效鉴定的候选者和参考指南；因此，开发一种用于奶牛品种生产和免疫特性的标记辅助选择（MAS）。

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Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows

The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.

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来源期刊

Journal of the Hellenic Veterinary Medical Society VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of the Hellenic Veterinary Medical Society (J Hellenic Vet Med Soc) is a quarterly peer-reviewed journal that publishes articles in all aspects of veterinary science and related disciplines. It is published by the Hellenic Veterinary Medical Society and is indexed in the Web of Science and in Scopus. There are no publication fees in the journal. Authors considering submitting manuscripts for evaluation and publication are requested to read carefully the instructions for authors and fully comply with them. Non-complying manuscripts may be returned to the corresponding author for formatting.