Weiqiang Wang, Bo He, Tingting Xiao, Minrui Xu, Bolin Liu, Yongshan Gao, Yanan Chen, Jie Li, Binghui Ge, Jinming Ma, Honghua Ge
{"title":"基于生物启发的淀粉样纤维水凝胶,具有工程可编程功能,可用于多种应用","authors":"Weiqiang Wang, Bo He, Tingting Xiao, Minrui Xu, Bolin Liu, Yongshan Gao, Yanan Chen, Jie Li, Binghui Ge, Jinming Ma, Honghua Ge","doi":"10.1002/adfm.202209441","DOIUrl":null,"url":null,"abstract":"<p>Natural proteins display organized hierarchical structures and tailored functionalities that cannot be achieved by synthetic approaches, highlighting the increased interest in developing protein-based materials. Protein self-assembly allows fabricating sophisticated supramolecular structures from relatively simple building blocks, a strategy naturally employed by amyloid proteins and intrinsically disordered proteins. However, the design of self-assembled bioinspired materials with multi functionalities is still challenging. Inspired by the natural self-assembly proteins (such as mussel foot proteins and amyloid proteins), a temperature-inducible engineering programable hydrogel-like amyloid nanostructure is developed by using a genetically modular fusion approach. The resulting hydrogel-like assemblies display outstanding adhesive capacity, high stability, and broad substrate universality. The employed SpyCatcher/SpyTag system allows modifying the hydrogel-like assemblies with any functional proteins of interest. Owing to their strong adhesive capacity and functional flexibility, such amyloid fibril-based hydrogel shows advantages in the immobilization of diverse enzymes for highly efficient biocatalysis, fabrication of multi-layered functional coatings, and construction of functionalized 3D scaffold for cell culture. Overall, a modular and straightforward approach is established to obtain a genetically programable nanostructure platform. The novel hydrogel-like assemblies described here may be potentially applied to but not limited to synthetic biology, surface/interface engineering, and tissue engineering.</p>","PeriodicalId":112,"journal":{"name":"Advanced Functional Materials","volume":null,"pages":null},"PeriodicalIF":18.5000,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioinspired Amyloid Fibril-Based Hydrogel with Engineering Programable Functionalities for Diverse Applications\",\"authors\":\"Weiqiang Wang, Bo He, Tingting Xiao, Minrui Xu, Bolin Liu, Yongshan Gao, Yanan Chen, Jie Li, Binghui Ge, Jinming Ma, Honghua Ge\",\"doi\":\"10.1002/adfm.202209441\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Natural proteins display organized hierarchical structures and tailored functionalities that cannot be achieved by synthetic approaches, highlighting the increased interest in developing protein-based materials. Protein self-assembly allows fabricating sophisticated supramolecular structures from relatively simple building blocks, a strategy naturally employed by amyloid proteins and intrinsically disordered proteins. However, the design of self-assembled bioinspired materials with multi functionalities is still challenging. Inspired by the natural self-assembly proteins (such as mussel foot proteins and amyloid proteins), a temperature-inducible engineering programable hydrogel-like amyloid nanostructure is developed by using a genetically modular fusion approach. The resulting hydrogel-like assemblies display outstanding adhesive capacity, high stability, and broad substrate universality. The employed SpyCatcher/SpyTag system allows modifying the hydrogel-like assemblies with any functional proteins of interest. Owing to their strong adhesive capacity and functional flexibility, such amyloid fibril-based hydrogel shows advantages in the immobilization of diverse enzymes for highly efficient biocatalysis, fabrication of multi-layered functional coatings, and construction of functionalized 3D scaffold for cell culture. Overall, a modular and straightforward approach is established to obtain a genetically programable nanostructure platform. The novel hydrogel-like assemblies described here may be potentially applied to but not limited to synthetic biology, surface/interface engineering, and tissue engineering.</p>\",\"PeriodicalId\":112,\"journal\":{\"name\":\"Advanced Functional Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":18.5000,\"publicationDate\":\"2023-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Functional Materials\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/adfm.202209441\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Functional Materials","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/adfm.202209441","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Bioinspired Amyloid Fibril-Based Hydrogel with Engineering Programable Functionalities for Diverse Applications
Natural proteins display organized hierarchical structures and tailored functionalities that cannot be achieved by synthetic approaches, highlighting the increased interest in developing protein-based materials. Protein self-assembly allows fabricating sophisticated supramolecular structures from relatively simple building blocks, a strategy naturally employed by amyloid proteins and intrinsically disordered proteins. However, the design of self-assembled bioinspired materials with multi functionalities is still challenging. Inspired by the natural self-assembly proteins (such as mussel foot proteins and amyloid proteins), a temperature-inducible engineering programable hydrogel-like amyloid nanostructure is developed by using a genetically modular fusion approach. The resulting hydrogel-like assemblies display outstanding adhesive capacity, high stability, and broad substrate universality. The employed SpyCatcher/SpyTag system allows modifying the hydrogel-like assemblies with any functional proteins of interest. Owing to their strong adhesive capacity and functional flexibility, such amyloid fibril-based hydrogel shows advantages in the immobilization of diverse enzymes for highly efficient biocatalysis, fabrication of multi-layered functional coatings, and construction of functionalized 3D scaffold for cell culture. Overall, a modular and straightforward approach is established to obtain a genetically programable nanostructure platform. The novel hydrogel-like assemblies described here may be potentially applied to but not limited to synthetic biology, surface/interface engineering, and tissue engineering.
期刊介绍:
Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week.
Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.