天然化合物在大鼠乳腺癌中的作用:山茱萸水提物的有益作用

Q3 Veterinary

Veterinarska stanica Pub Date : 2023-06-27 DOI:10.46419/vs.55.1.3

Tiago Azevedo, Jéssica Silva, F. Peixoto, A. Silvestre-Ferreira, A. Gama, F. Seixas, T. Finimundy, Lilian Barros, Manuela Matos, Paula A. Oliveira, A. Faustino-Rocha, Abigaël Valada, Lara Anjos, Tânia Moura, Rafaela Ferreira, Marlene Santos, M. J. Pires, M. Neuparth

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引用次数: 0

摘要

乳腺癌是女性中诊断最多的癌症，也是世界范围内导致死亡的主要原因。圣莲是一种具有多种健康益处的植物，包括抗癌和抗糖尿病的特性。本研究旨在探讨沙棘水提物(SCE)对乳腺癌动物模型的化学预防作用。将28只4周龄雌性Wistar大鼠分为对照组、mnu诱导组(IND)、SCE补充组(SCE)和SCE+IND组。饮用水中随意添加SCE (12.72 mg/kg体重)。在50日龄时通过腹腔注射MNU。每周监测体重、食物/饮料摄入量、人道终点和乳腺肿瘤数量。MNU给药20周后，用麻醉过量处死动物，并进行尸检。血液样本用于测定血细胞计数和血清生化分析，同时分析肾脏和肝脏样本的氧化应激。收集肿瘤样本进行基因表达和组织学研究。用LC-MS分析了SCE的化学成分，共含有19种酚类化合物，其中含量最多的是杨梅素- o -葡萄糖醛酸和1,3- o -二咖啡酰奎宁酸。IND组中有2只动物因超过人道终点限制而被处死。补充SCE延缓了乳腺肿瘤的发展，减少了肿瘤的体积和重量。SCE对血液学参数有积极影响，特别是中性粒细胞-淋巴细胞比率(P=0.026)。除肌酐激酶MB和氧化应激标志物外，两组血清生化指标无显著差异。基因表达分析显示，SCE+IND肿瘤中VEGF表达水平显著降低(P=0.0158)。这些发现表明，SCE值得进一步研究，以确定单个化合物，并了解其在癌症发展过程中对动物模型的影响。

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The role of natural compounds in rat mammary cancer: the beneficial effects of Santolina chamaecyparissus L. aqueous extract

Breast cancer is the most diagnosed cancer among women, and a leading cause of death worldwide. Santolina chamaecyparissus L. is a plant with multiple health benefits, including anticancer and anti-diabetic properties. This study aimed to assess the chemopreventive effects of S. chamaecyparissus aqueous extract (SCE) in an animal model of mammary cancer. A total of 28 four-week-old female Wistar rats were divided into four groups: control, MNU-induced (IND), SCE-supplemented (SCE), and SCE+IND. SCE was added to drinking water (12.72 mg/kg body weight) ad libitum. MNU was administered via the intraperitoneal route at 50 days of age. Weekly monitoring of body weight, food/drink intake, humane endpoints, and number of mammary tumours were recorded. Twenty weeks after MNU administration, animals were sacrificed by anaesthetic overdose and a necropsy was performed. Blood samples were used to determine blood count and serum biochemistry analysis, while kidney and liver samples were analysed for oxidative stress. Tumour samples were collected for gene expression and histology studies. SCE chemical composition was analysed by LC-MS and contained 19 phenolic compounds, with the most abundant being myricetin-O-glucuronide and 1,3-O-dicaffeoylquinic acid. Two animals in the IND group were sacrificed due to exceeding the humane endpoint limits. SCE supplementation delayed mammary tumour development, reducing its volume and weight. SCE had a positive impact on haematological parameters, particularly the neutrophil-lymphocyte ratio (P=0.026). No significant differences were observed in serum biochemistry, except for creatinine kinase MB, or in oxidative stress markers. Gene expression analysis showed significantly reduced VEGF expression levels (P=0.0158) in tumours from SCE+IND. These findings suggest that SCE is deserving of further study to identify the individual compounds and to understand its influence on animal models during cancer development.

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来源期刊

Veterinarska stanica Veterinary-Veterinary (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The goal of the journal is to provide an international platform for the publication of articles in the fields of veterinary and animal sciences, and biotechnology. The content of the journal is particularly dedicated to veterinary practitioners, but also to veterinary scientists and university professors, to encourage them to share their knowledge and experience on this platform. Manuscripts submitted to the journal may include: original scientific papers, review articles, short communications, professional articles, case reports, conference reports and literary records and reviews of new book either in Croatian or English languages.