脐带组织移植对辐射致大鼠学习记忆损伤的保护作用及机制

中华行为医学与脑科学杂志 Pub Date : 2020-01-20 DOI:10.3760/CMA.J.CN371468-20190425-00287

Zejun Zhu, Yang Zhao, A. Zhang, Nai-yao Chen, Hui Zhao, D. Bai, Xiujun Zhang

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引用次数: 0

摘要

目的探讨脐带组织移植对辐射致大鼠学习记忆损伤的保护作用及其机制。方法将60只SD大鼠随机分为3组，每组20只:对照组、模型组(全脑x线照射，剂量20 Gy)和治疗组(全脑x线照射，剂量20 Gy +脐带组织移植)。照射后第14、28天采用水迷宫法观察大鼠体质量变化，HE染色观察海马神经元状态，Western blot检测海马组织中NF-κB通路相关蛋白及IL-6的表达。采用SPSS 17.0进行描述性分析和假设检验。结果(1)第28天，水迷宫实验中，治疗组大鼠逃避潜伏期显著高于对照组，低于模型组(对照组:(11.77±3.02)s，模型组:(23.75±3.27)s，治疗组:(18.49±2.32)s;t=3.940， -2.943, P均<0.05);治疗组小鼠交叉平台个数显著低于对照组，显著高于模型组(对照组:(7.20±0.84)个，模型组(3.60±1.14)个，治疗组(5.00±1.00)个;t=-3.773, 2.064, P均<0.05)。(2)HE染色显示，对照组神经元排列有序，胞质透明。模型组神经元排列紊乱，胞体呈三角形或不规则收缩。治疗组神经元数量明显少于模型组。(3)第14天，治疗组TLR4相对表达量显著高于对照组，低于模型组(对照组:(0.69±0.03)，模型组:(1.06±0.11)，治疗组:(0.90±0.04);t=7.275， -2.368, P均<0.05)。治疗组NF-κB p65的相对表达量显著高于对照组，低于模型组(对照组:(1.67±0.12)，模型组:(2.08±0.06)，治疗组:(1.93±0.08);t=3.236， -2.684, P均<0.05)。治疗组IL-6的相对表达量显著高于对照组，低于模型组(对照组:0.77±0.08)，模型组:1.12±0.07，治疗组:0.95±0.06);t=3.274， -3.495, P均<0.05)。治疗组Bcl-2 / Bax的相对表达量显著低于对照组，高于模型组(对照组:(1.40±0.52)，模型组:(0.48±0.06)，治疗组:(0.72±0.0 3);t=-2.263, 6.350, P均<0.05)。IL-6和Bcl-2 / Bax蛋白在第28天的表达趋势与第14天相同。结论脐带组织移植可改善放疗所致的学习记忆障碍，这可能与抑制放疗引起的炎症有关。关键词:脐带;脐带间充质干细胞;放射治疗;炎症;学习与记忆

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Protective effect and mechanisms of umbilical cord tissue transplantation on radiation-induced learning and memory impairment in rats

Objective To investigate the protective effect and mechanisms of umbilical cord tissue transplantation on radiation-induced learning and memory impairment in rats. Methods Sixty SD rats were randomly divided into three groups with 20 in each group: control group, model group (whole brain X-ray irradiation, dose 20 Gy) and treatment group (whole brain X-ray irradiation, dose 20 Gy + umbilical cord tissue transplantation). The changes of body mass were observed, and the learning and memory of rats were observed by water maze test on the 14th and 28th day after irradiation, the neuron state of hippocampus was observed by HE staining, and the expressions of NF-κB pathway related proteins and IL-6 in hippocampus were detected by Western blot.Descriptive analysis and hypothesis testing were processed by SPSS 17.0. Results (1) On the 28th day, the escaping latency in the water maze experiment of the treatment group was significantly higher than that of the control group and lower than that of the model group (control group: (11.77±3.02) s, model group: (23.75±3.27)s, treatment group: (18.49±2.32)s; t=3.940, -2.943, both P<0.05); the number of crossing platform in the treatment group was significantly lower than that in the control group and higher than that in the model group (control group: (7.20±0.84), model group (3.60±1.14 ), treatment group (5.00±1.00); t=-3.773, 2.064, both P<0.05). (2)HE staining showed that the neurons in the control group were arranged orderly and the cytoplasm was transparent.The neurons in the model group were arranged disorderly and the contraction of the cell body was triangular or irregular.The number of neurons in the treatment group was less than that in the model group. (3) On the 14th day, the relative expression of TLR4 in the treatment group was significantly higher than that in the control group and lower than that in the model group (control group: (0.69±0.03), model group: (1.06±0.11), treatment group: (0.90±0.04); t=7.275, -2.368, both P<0.05). The relative expression of NF-κB p65 in the treatment group was significantly higher than that in the control group and lower than that in the model group (control group: (1.67±0.12), model group: (2.08 ±0.06), treatment group: (1.93±0.08); t=3.236, -2.684, both P<0.05). The relative expression of IL-6 in the treatment group was significantly higher than that in the control group and lower than that in the model group (control group: (0.77±0.08), model group: (1.12±0.07), treatment group: (0.95±0.06); t=3.274, -3.495, both P<0.05). The relative expression of Bcl-2 / Bax in the treatment group was significantly lower than that in the control group and higher than that in the model group (control group: (1.40±0.52), model group: (0.48±0.06), treatment group: (0.72±0.0 3); t=-2.263, 6.350, both P<0.05). The expression trend of IL-6 and Bcl-2 / Bax protein on the 28th day was the same as that on the 14th day. Conclusion Cord tissue transplantation can improve the learning and memory impairment caused by radiotherapy, which may be related with the inhibition of inflammation caused by radiotherapy. Key words: Umbilical cord; Umbilical cord mesenchymal stem cells; Radiation therapy; Inflammation; Learning and memory

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中华行为医学与脑科学杂志

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期刊介绍： "Chinese Journal of Behavioral Medicine and Brain Science" (CN 37-1468/R, ISSN 1674-6554) is a national academic journal under the supervision of the National Health Commission, sponsored by the Chinese Medical Association and Jining Medical College. The journal was founded in June 1992 and was formerly known as "Chinese Journal of Behavioral Medicine" (1992-1993) and "Chinese Behavioral Medical Science" (1994-2008). In 2009, it was renamed "Chinese Journal of Behavioral Medicine and Brain Science" with the approval of the State Administration of Press, Publication, Radio, Film and Television. The purpose of "Chinese Journal of Behavioral Medicine and Brain Science" is to implement the health and health policies of the Party and the State, implement the principle of combining theory with practice and popularization and improvement, and reflect the major progress in the theory and practical application of behavioral medicine and brain science in my country. It publishes academic papers and scientific research results in the field of behavioral medicine and brain science in my country, and has columns such as monographs/reviews, basic research, clinical research, health prevention, methods and techniques, psychological behavior and evaluation, and systematic evaluation.