酵母细胞中4-苯基丁酸对内质网相关室的分散作用。

IF 2 4区 生物学 Q4 CELL BIOLOGY
T. Mai, Yuki Ishiwata‐Kimata, Q. Le, Hiroyuki Kido, Y. Kimata
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引用次数: 3

摘要

在酵母细胞中,一些异常的多膜跨越蛋白不会被运输到细胞表面,而是形成并积聚在内质网(ER)衍生的亚室中,称为内质网相关室(ERACs),荧光显微镜下可以观察到这些小点。在这里,我们发现细胞表面蛋白Pma1的突变体Pma1-2308在erac中积累,以及在酵母细胞中异源表达的哺乳动物囊性纤维化跨膜传导调节因子(CFTR)。Pma1-2308和CFTR位于同一erac上。我们还注意到,用4-苯基丁酸(4-PBA)处理细胞会破坏Pma1-2308和CFTR形成的ERAC,这表明4-PBA在酵母细胞中发挥了类似伴侣的功能。有趣的是,与典型内质网应激源tunicamycin诱导的内质网应激不同,Pma1-2308诱导的内质网应激被4-PBA加重。我们假设这一观察结果表明了erac的有益方面,因此提出erac是通过异常跨膜蛋白的聚集形成的,并作为多种erac形成蛋白的积累位点进行隔离。关键词:蛋白聚集,细胞器,未折叠蛋白反应,内质网应激,4-PBA
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dispersion of endoplasmic reticulum-associated compartments by 4-phenyl butyric acid in yeast cells.
In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy. Here we show that a mutant of the cell surface protein Pma1, Pma1-2308, was accumulated in the ERACs, as well as the heterologously expressed mammalian cystic fibrosis transmembrane conductance regulator (CFTR), in yeast cells. Pma1-2308 and CFTR were located on the same ERACs. We also note that treatment of cells with 4-phenyl butyric acid (4-PBA) compromised the ERAC formation by Pma1-2308 and CFTR, suggesting that 4-PBA exerts a chaperone-like function in yeast cells. Intriguingly, unlike ER stress induced by the canonical ER stressor tunicamycin, ER stress that was induced by Pma1-2308 was aggravated by 4-PBA. We assume that this observation demonstrates a beneficial aspect of ERACs, and thus propose that the ERACs are formed through aggregation of aberrant transmembrane proteins and work as the accumulation sites of multiple ERAC-forming proteins for their sequestration. Key words: protein aggregation, organelle, unfolded protein response, ER stress, 4-PBA.
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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