{"title":"LncRNA-p21介导Wnt/β-Catenin信号通路调控胃癌转移的作用及机制","authors":"Peng Liu, Haizhe Chang, Xinyu Peng, Xiuqin Zhang, Jianjun Zhang, Jiusong Luan","doi":"10.26689/jcnr.v7i3.4862","DOIUrl":null,"url":null,"abstract":"Objective: To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway. Methods: Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration, invasion, cell morphology and proliferation. Besides. Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation. Wnt/β-catenin signaling pathway was validated using Li-C1. Results: Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology, low invasion, or spindle-shaped morphology. LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro, and Wnt/β-catenin signaling pathway mediated the proliferation, invasion, and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells. Conclusion: LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo, and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.","PeriodicalId":64151,"journal":{"name":"临床护理研究","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Role and Mechanism of LncRNA-p21 in Regulating Gastric Cancer Metastasis by Mediating Wnt/β-Catenin Signaling Pathway\",\"authors\":\"Peng Liu, Haizhe Chang, Xinyu Peng, Xiuqin Zhang, Jianjun Zhang, Jiusong Luan\",\"doi\":\"10.26689/jcnr.v7i3.4862\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway. Methods: Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration, invasion, cell morphology and proliferation. Besides. Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation. Wnt/β-catenin signaling pathway was validated using Li-C1. Results: Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology, low invasion, or spindle-shaped morphology. LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro, and Wnt/β-catenin signaling pathway mediated the proliferation, invasion, and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells. Conclusion: LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo, and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.\",\"PeriodicalId\":64151,\"journal\":{\"name\":\"临床护理研究\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"临床护理研究\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.26689/jcnr.v7i3.4862\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"临床护理研究","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.26689/jcnr.v7i3.4862","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Role and Mechanism of LncRNA-p21 in Regulating Gastric Cancer Metastasis by Mediating Wnt/β-Catenin Signaling Pathway
Objective: To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway. Methods: Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration, invasion, cell morphology and proliferation. Besides. Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation. Wnt/β-catenin signaling pathway was validated using Li-C1. Results: Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology, low invasion, or spindle-shaped morphology. LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro, and Wnt/β-catenin signaling pathway mediated the proliferation, invasion, and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells. Conclusion: LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo, and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.
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