转录组测序揭示了太平洋鲍鱼对急性热应激的反应

IF 1.9 4区 农林科学 Q2 FISHERIES
Zhou Wu, Yumin Yang, Liqing Zhou, Changfeng Chi, Xiujun Sun, Biao Wu, Zhihong Liu, Yan Wang
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引用次数: 0

摘要

太平洋鲍鱼是一种经济上重要的冷水贝类。随着养殖的广泛,高温已成为夏季太平洋鲍鱼高死亡率的关键非生物因素,特别是在中国南方。为了进一步了解太平洋鲍鱼热应激反应的分子调控机制,进一步分析其热适应能力,我们将太平洋鲍鱼分别置于28°C下的急性热应激6、24和48小时。与没有热刺激的对照组相比,在热应激6、24和48小时后,太平洋鲍鱼分别鉴定出2,213、2,337和1,420个差异表达基因(deg)。这些deg在蛋白质折叠和伴侣介导的蛋白质折叠过程、抗原加工和递呈以及内质网途径的蛋白质加工中显著富集。热应激后,多种分子伴侣蛋白的表达显著上调。通过上调与内质网相关降解(ERAD)途径相关的分子伴侣的表达来维持细胞稳态可能是太平洋鲍鱼抵御热应激的核心。此外,TLR信号通路toll样受体6 (TLR6)和髓样分化因子88 (MyD88)基因的表达也被诱导。因此,在热激后,太平洋鲍鱼可能通过上调免疫相关基因激活免疫防御系统来抵御病原菌的入侵。本研究结果不仅有助于深入了解太平洋鲍鱼的热应激调控,而且为耐热鲍鱼的分子遗传育种提供了基础数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome Sequencing Reveals the Response to Acute Thermal Stress in the Pacific Abalone, Haliotis discus hannai
The Pacific abalone is an economically important cold-water shellfish. With its widespread culture, high temperature has become a key abiotic factor for the high mortality of Pacific abalone in summer, particularly in the south of China. To understand the molecular regulatory mechanisms of thermal stress response in Pacific abalone for further analyzing its heat adaptive capacity, we subjected Pacific abalone to acute heat stress at 28°C for 6, 24, and 48 hr, respectively. A total of 2,213, 2,337, and 1,420 differentially expressed genes (DEGs) were identified in Pacific abalone after 6, 24, and 48 hr of thermal stress, respectively, as compared with the control group without heat stimulation. These DEGs were significantly enriched in protein folding and chaperone-mediated protein folding process, antigen processing and presentation, and protein processing in endoplasmic reticulum pathways. The expression of numerous molecular chaperones was significantly upregulated after thermal stress. Maintaining cellular homeostasis through the upregulated expression of molecular chaperones associated with endoplasmic reticulum-associated degradation (ERAD) pathways might be central to the defense of Pacific abalone against thermal stress. In addition, the expression of Toll-like receptor 6 (TLR6) and myeloid differentiation factor 88 (MyD88) genes of the TLR signaling pathway was also induced. Therefore, the Pacific abalone might activate the immune defense system to resist the invasion of pathogenic bacteria by upregulating immune-related genes after thermal challenges. Our results not only contribute to a deeper understanding of heat stress regulation in the Pacific abalone but also provide basic data for molecular genetic breeding of heat-resistant abalone.
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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