苦瓜提取物中小檗碱含量测定方法的建立与验证

IF 0.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Ashwini Tonape, Vrushali Neve, Jui Darbhe, Vrushali Bhalchim
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引用次数: 0

摘要

背景:虽然已经发现几种抗糖尿病药物可以降低血糖水平,但目前市场上销售的抗糖尿病药物也有许多负面影响。然而,有一些药用植物具有抗糖尿病作用,副作用低或无副作用。在葫芦科植物中,苦瓜(Momordica dioica)是一种雌雄异株攀缘草本植物。它含有许多植物成分。其中之一是苯基异喹啉生物碱的季铵盐,称为小檗碱,具有多种药用特性。本研究主要探讨小檗碱在薯蓣属植物中的抗糖尿病活性。本研究旨在建立一种快速、准确、准确、特异的高效液相色谱法测定苦瓜提取物中小檗碱的方法。方法/结论/意义:为了快速检测小檗碱的含量,建立并验证了HPTLC方法。在HPTLC 60f254铝板上,预包被硅胶,以甲醇、乙酸乙酯、甲苯和甲酸(2:4:3:5 . 0)为溶剂进行色谱分离。检测波长为348 nm。小檗碱的Rf值为0.44±5%。黄连素在400ng/波段浓度范围内呈线性关系。小檗碱的LOD和LOQ分别为0.0096ng/band和0.0293ng/ band。小檗碱的平均回收率为(0.041)。该方法的精密度、准确度、线性、稳健性和特异性均按照ICH指南进行验证。所发现的方法可用于定期分析草药制剂中的小檗碱,以进行质量控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development and Validation of Analytical Method for Estimation of Berberine in Herbal Extract of Momordica Dioica
Background:While several anti-diabetic medications have been found to lower blood glucose levels, there are numerous negative effects connected with the use of currently marketed anti-diabetic medications.However, there are medicinal plants with anti-diabetic effects with low or no side effects. Among the Cucurbitaceae family of plants, Momordica dioica is a dioecious climbing herb. It contains many phytoconstituents.One of it is a quaternary ammonium salt of benzyl-isoquinoline alkaloid called as berberine exhibiting a wide variety of pharmaceutical properties.In the current investigation, the activities of berberine for its anti-diabetic potential in the Momorica Dioica plant.The goal of the recent effort is to establish and validate an HPTLC method that is quick, accurate, exact, and specific for determining berberine from herbal extract of Momordica dioica. Methodology/Conclusions/significance: For a quick examination to determine the amount of berberine, a (HPTLC) process was created as well as validated.On HPTLC aluminium plate 60 F254,precoated with silica gel  chromatographic separation was accomplished using the solvents: methanol ,ethyl acetate, toluene and formic acid (2:4:3:0.5). A wavelength of 348 nm was used for detection. Rf was found to be 0.44±5% for berberine. There was linearity for berberine in the 400ng/band concentration range.The LOD and LOQ was found to be0.0096ng/band and 0.0293ng/ band for Berberine.The average berberine recovery rate was (0.041).The method's precision, accuracy, linearity, robustness, and specificity were all authenticated in accordance with ICH guidelines. The discovered approach can be used to regularly analyse Berberine in herbal formulations for quality control.
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来源期刊
International Journal of Ayurvedic Medicine
International Journal of Ayurvedic Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
50.00%
发文量
87
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