韩国人类孢子虫病的分子鉴定

Q4 Medicine

Journal of Mycology and Infection Pub Date : 2019-06-30 DOI:10.17966/JMI.2019.24.2.45

Hye Ri Kim, D. Shin, J. Lee, J. Choi

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引用次数: 2

摘要

背景：孢子丝菌病是由申克孢子丝复合体引起的一种常见的深部真菌病。直到2016年，还没有对这些真菌进行过分子研究，所有被纳入的菌株都被报道为申克酵母。然而，在中国东北部、日本和印度进行的调查显示，球形S.globosa是最普遍的孢子丝菌物种，而严格意义上的申克氏S.schenckii报道很少。目的：调查韩国申克克氏菌中孢子丝菌病的准确流行致病种。方法：我们从真菌采集中心或私人收藏中分离出在韩国流行的孢子丝菌菌株，并回顾了该地区菌株的分子研究文献。我们发现了5株申克氏链霉菌（1998-2016）和3株球状链霉菌（2016-2018）。将这些菌株的核糖体DNA内部转录间隔区（ITS）序列与申克氏酵母复合物菌株的序列进行了比较。结果：8株菌株的核糖体ITS序列与球形链霉菌的核糖体ITS完全一致。未发现严格意义上的申克氏杆菌。此外，Ishizaki等人（2004年）发表的一项关于韩国申克氏梭杆菌分子分析的研究表明，这八个菌株属于线粒体亚型B组（球形梭杆菌）。因此，在韩国申克氏链霉菌复合体中检测的所有16个菌株都被确定为球状链霉菌。结论：总之，球形S.globosa是1998年至2018年间报告的韩国孢子丝菌病病例中的致病种。根据我们的分析，球形S.globosa而不是申克氏S.schenckii可能是韩国的优势物种。

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Molecular Identification of Human Sporotrichosis in Korea

Background: Sporotrichosis is a common deep mycosis caused by the Sporothrix schenckii complex. Until 2016, no molecular studies had been conducted on these fungi, and all the included strains were reported as S. schenckii. However, investigations conducted in northeast China, Japan, and India revealed that S. globosa was the most prevalent Sporothrix species, whereas S. schenckii sensu stricto was reported very rarely. Objective: To investigate the accurate prevalent causative species of sporotrichosis among strains reported as S.schenckii in Korea. Methods: We isolated strains of Sporothrix spp. Prevalent in Korea from fungus collection centers or private collections and reviewed the available literature on molecular studies of strains from this region. We found five S. schenckii (1998-2016) and three S. globosa (2016-2018) strains. Ribosomal DNA internal transcribed spacer (ITS) sequences of these strains were compared with those of the S. schenckii complex strains. Results: The ribosomal ITS sequences of the eight strains were 100% identical with that of S. globose. No S. schenckii sensu stricto was found. In addition, a study on the molecular analysis of Korean S. schenckii published by Ishizaki et al. (2004) demonstrated that the eight strains were of the mitochondrial subtype group B (S. globosa). Thus, all the 16 strains examined within the Korean S. schenckii complex were determined to be S. globosa. Conclusion: In summary, S. globosa is the causative species within the tested Korean sporotrichosis cases reported between 1998 and 2018. Based on our analyses, S. globosa, and not S. schenckii, may be the predominant species in Korea.

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来源期刊

Journal of Mycology and Infection Medicine-Infectious Diseases

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： The Journal of mycology and infection (Acronym: JMI, Abbreviation: J Mycol Infect) aims to publish articles of exceptional interests in the field of medical mycology. The journal originally was launched in 1996 as the Korean Journal of Medical Mycology and has reformed into the current state beginning on March of 2018. The contents of the journal should elucidate important microbiological fundamentals and provide qualitative insights to respective clinical aspects. JMI underlines the submission of novel findings and studies in clinical mycology that are enriched by analyses achieved through investigative methods. The journal should be of general interests to the scientific communities at large and should provide medical societies with advanced breadth and depth of mycological expertise. In addition, the journal supplements infectious diseases in adjunct to the field of mycology to address a well-rounded understanding of infectious disorders. The Journal of mycology and infection, which is issued quarterly, in March, June, September and December each year, published in English. The scope of the Journal of mycology and infection includes invited reviews, original articles, case reports, letter to the editor, and images in mycology. The journal is compliant to peer-review/open access and all articles undergo rigorous reviewing processes by our internationally acknowledged team of editorial boards. The articles directed to publication should encompass in-depth materials that employ scholastic values of mycology and various infectious diseases. Articles responding to critical methodology and outcomes which have potential to enhance better understanding of mycology and infectious diseases are also suitable for publication.