蓝色和红色激光激活卡图克叶片叶绿素(Sauropus androgynus (L) Merr)对减少聚集菌放线菌和粪肠球菌生物膜的效果

Q3 Medicine
P. Permatasari, S. D. Astuti, A. K. Yaqubi, E. A. W. Paisei, .. Pujiyanto, Nasrul Anuar
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引用次数: 0

摘要

本研究考察了雌雄同体索罗pus (L) Merr(一种卡图克叶片叶绿素光敏剂)对聚集菌放线菌和粪肠球菌生物膜的抑制作用。采用红蓝二极管激光器作为光源。将样品分为4组:阴性对照组、阳性对照组、蓝色激光处理组(B)和红色激光处理组(R),分别添加和不添加1.6 mg/ml的木参叶叶绿素,激光能量暴露密度分别为2.5 J/cm2、5 J/cm2、7.5 J/cm2和10 J/cm2。采用ELISA和方差分析对激光照射和叶绿素光敏剂进行检测。在能量密度为10 J/cm2时,各处理组细菌死亡率最佳。即在放线菌聚集菌生物膜阴性组中,蓝色二极管激光的死亡率为73.30%,红色二极管激光的死亡率为63.25%。在阳性组中,蓝色二极管激光的死亡率为86.12%,红色二极管激光的死亡率为83.29%。在粪肠球菌生物膜中,阴性组蓝色二极管激光死亡率为67.78%,红色二极管激光死亡率为75.33%,阳性组蓝色二极管激光死亡率为71.71%,红色二极管激光死亡率为86.41%。暴露在蓝色和红色二极管激光下会激活卡图克树叶中的叶绿素,杀死细菌并减少生物膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm
In this study, the efficacy of using Sauropus androgynus (L) Merr, a katuk leaf chlorophyll photosensitizer, to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm was investigated. A red and blue diode laser is used as the light source. The sample was split into four groups: a negative control group, a positive control group, a blue laser treatment group (B), and a red laser treatment group (R), both with and without the addition of katuk leaf chlorophyll 1.6 mg/ml, and with varying densities of laser energy exposure of 2.5 J/cm2, 5 J/cm2, 7.5 J/cm2, and 10 J/cm2. Laser exposure and chlorophyll photosensitizer were tested using ELISA and ANOVA. At an energy density of 10 J/cm2, the optimal bacterial mortality rate was obtained in each treatment group. Namely, in the Aggregatibacter actinomycetemcomitans biofilm, the negative group, the number of deaths was 73.30% using a blue diode laser and 63.25% using a red diode laser. In the positive group, the number of deaths was 86.12% using a blue diode laser and 83.29% using a red diode laser. In the Enterococcus faecalis biofilm, in the negative group, the number of deaths was 67.78% using the blue diode laser and 75.33% using the red diode laser, and in the positive group, the number of deaths was 71.71% using the blue diode laser and 86.41 using a red diode laser. Exposure to blue and red diode lasers activates chlorophyll in katuk leaves, killing bacteria and reducing biofilms.
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来源期刊
Biomedical Photonics
Biomedical Photonics Medicine-Surgery
CiteScore
1.80
自引率
0.00%
发文量
19
审稿时长
8 weeks
期刊介绍: The main goal of the journal – to promote the development of Russian biomedical photonics and implementation of its advances into medical practice. The primary objectives: - Presentation of up-to-date results of scientific and in research and scientific and practical (clinical and experimental) activity in the field of biomedical photonics. - Development of united Russian media for integration of knowledge and experience of scientists and practitioners in this field. - Distribution of best practices in laser medicine to regions. - Keeping the clinicians informed about new methods and devices for laser medicine - Approval of investigations of Ph.D candidates and applicants.
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