菊苣水提取物在脑缺血再灌注损伤相关的大脑中动脉闭塞性卒中模型中的神经保护潜力。

Beenish Sohail, N. Khan
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引用次数: 0

摘要

背景:在全球范围内,中风是人类感觉和运动障碍的难以捉摸的基础。研究表明,多种草药化合物与药物疗法结合使用,对缺血性中风的治疗和初级预防具有积极的治疗潜力。目前的研究旨在确定在脑中动脉闭塞(MCAO)前和后缺血再灌注损伤模型中使用猴头菌(HE)水提取物的神经保护潜力。方法:54只Wistar白化大鼠(200-250克;10-12周)被分为四个实验组(n=9)。第一组(对照组);第二组(假手术);第三组(MCAO)MCAO再灌注20-30分钟,再灌注24小时;第四组分为三组,即第一组(300mg/kg BWT的AEHE给药7天)、第二组(300mmg/kg BWT的AE给药7天后,再进行MCAO)、第三组(在MCAO诱导第4小时给予300mg/kg的AEHE)。保存血液和大脑、肝脏和肾脏组织样本,并评估血脂水平、肝肾水平、c-反应蛋白、血糖和组织抗氧化水平的变化。对选定的组织进行了组织病理学研究。结果:MCAO诱导可显著改变肾肝功能、c-RP水平和动物组织抗氧化(CAT、SOD、GSH)酶水平。然而,两个亚组(MCAO前和MCAO后HE)的HE提取物显著降低了脂质组、葡萄糖水平和肾-肝参数。HE能有效调节大脑、肝脏和肾脏匀浆中的抗氧化酶。在MCAO前和MCAO后HE组中,组织的组织病理学结构得以保留。结论:在缺血再灌注损伤的大鼠脑卒中模型中,每日食用计算量的HE提取物可显著降低与MCAO相关的生化变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neuroprotective Potential of Hericium Erinaceus aqueous extract utilization in ischemia-reperfusion injury related middle cerebral artery occlusion stroke model.
Background: Globally, stroke is the elusive basis of sensory and motor impairments in humans. Multiple herbal compounds have been shown in studies to have positive therapeutic potential when used in conjunction with pharmaceutical therapies for the treatment and primary prevention of ischemic stroke. Current research investigation is planned to determine the neuro-protective potential of Hericium Erinaceus (HE) aqueous extract utilization in pre and post-middle cerebral artery occlusion (MCAO) model of ischemic–reperfusion injury. Methodology: Fifty-four Wistar albino rats (200-250 gms; 10-12 weeks) were divided into four experimental groups (n=9). Group I (control); Group II (sham); Group III (MCAO) MCAO for 20-30 min then 24 hours of reperfusion; Group IV was split into three groups as subgroup I (300 mg/kg BWT of AEHE given for 7 days), subgroup II (300 mg/kg BWT of AEHE given for 7 days and then MCAO), subgroup III (at the 4th hour of MCAO induction 300mg/kg of AEHE was given). Blood and cerebral, hepatic, and renal tissue samples were preserved and evaluated for modifications in plasma lipids levels, liver-kidney levels, c-reactive protein, blood glucose, and tissue antioxidant levels. A histopathological study was done over the selected tissues. Results: MCAO induction significantly alters the reno-hepatic profiles, c-RP levels, and animal tissue antioxidant (CAT, SOD, GSH) enzymes levels. However, HE-extract in both subgroups (Pre MCAO HE and Post MCAO HE) has significantly reduced lipid panel, glucose levels, and reno-hepatic parameters significantly. HE has effectively regulated antioxidant enzymes in cerebral, hepatic, and renal homogenates. In Pre-MCAO HE and Post-MCAO HE groups, the histopathological architecture of tissues is preserved. Conclusion: Daily dietary consumption of HE extract in calculated quantity significantly reduces the biochemical changes related to MCAO in a rat stroke model in ischemia-reperfusion injury.
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