E. R. Janusckiewicz, Elisamara Raposo, B. R. Martins, M. A. Magalhães, A. R. Panosso, G. M. Melo, A. C. Ruggieri
{"title":"饲料供应下腕鱼牧草土壤酶活性的测定","authors":"E. R. Janusckiewicz, Elisamara Raposo, B. R. Martins, M. A. Magalhães, A. R. Panosso, G. M. Melo, A. C. Ruggieri","doi":"10.17523/bia.2019.v76.e1460","DOIUrl":null,"url":null,"abstract":"The soil enzymatic activity reflects the microorganism’s activity that decompose the organic matter and release nutrients to the plants. The objective was to evaluate the soil enzymatic activity in the Brachiaria pastures managed under the forage allowances of 4, 7, 10 and 13 kg MS/100 kg BW. The grazing method adopted was mob-stocking, with four grazing cycles (between December 2008 and February 2009). We also performed a sampling in June 2009 for better characterization, as the mineralization of soil organic matter occurs in the long term. The experimental design was completely randomized, with three replications and time subdivided plots. Forage allowances did not maintain a pattern of the activity of the enzyme’s dehydrogenase, protease, arylsulfatase and cellulase. Among the cv., there was a greater (P<0.05) activity of dehydrogenase and arylsulfatase in the cv. Marandu. Activity of dehydrogenase observed was 169,90; 189,19; 185,65; 247,74; and 126,00 μg TFF/g soil, and arylsulfatase was 29,69; 31,19; 38,14; 34,22; and 38,33 μg paranitrofenol/g soil, respectively to 1, 21, 42, 63, and 183 evaluation days. The protease activity was higher (P<0.05) for cv. Marandu, with the exception of evaluation day 42, where the highest activity was for cv. Mulato (82,38 μg tirosina/g soil). For cv. Marandu, the protease activity obtained was 80,49; 108,23; 86,01; and 134,71 μg tirosina/g soil solo, respectively to 1, 21, 63, and 183 evaluation days. Marandu cultivar is the most indicate to promove better conditions for the microorganisms responsible to the enzymatic activity of dehydrogenase, arylsulfatase, and protease. It can be inferred that a longer study time is required in order to increase the specificity of the forage allowances in relation to the behavior of the enzyme activity.","PeriodicalId":43616,"journal":{"name":"BOLETIM DE INDUSTRIA ANIMAL","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Atividade enzimática do solo de pastos de Brachiaria manejados sob ofertas de forragem\",\"authors\":\"E. R. Janusckiewicz, Elisamara Raposo, B. R. Martins, M. A. Magalhães, A. R. Panosso, G. M. Melo, A. C. Ruggieri\",\"doi\":\"10.17523/bia.2019.v76.e1460\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The soil enzymatic activity reflects the microorganism’s activity that decompose the organic matter and release nutrients to the plants. 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引用次数: 2
摘要
土壤酶活性反映了微生物分解有机质和向植物释放养分的活性。目的是评价在饲料量为4、7、10和13 kg MS/100 kg BW的条件下,放牧放牧放牧的Brachiaria牧场土壤酶活性。放牧方式为暴民放养,共4个放牧周期(2008年12月- 2009年2月)。我们还在2009年6月进行了采样,以便更好地表征,因为土壤有机质的矿化是长期发生的。试验设计完全随机化,3个重复,按时间细分。饲料用量对酶的脱氢酶、蛋白酶、芳基硫酸酯酶和纤维素酶活性没有影响。在简历中。脱氢酶和芳基硫酸酯酶活性显著高于对照(P<0.05)。Marandu。脱氢酶活性分别为169、90;189年,19个;185年,65年;247年,74年;12.6万μg TFF/g土壤,芳基磺化酶为29,69;31日,19日;38岁,14;34岁的22;对硝基酚/g土壤分别为38、33 μg、1、21、42、63、183评价天。血清蛋白酶活性显著高于对照组(P<0.05)。除评价第42天外,马兰度(Marandu)的cv活性最高。Mulato (82,38 μg tirosina/g soil)。的简历。得到的蛋白酶活性分别为80,49;108年,23个;86年,01;1、21、63、183评价d时,土壤单质tirosina含量分别为134、71 μg /g。马兰度品种对脱氢酶、芳基硫酸酯酶和蛋白酶活性相关的微生物提供了较好的生长条件。由此可以推断,为了增加与酶活性行为相关的饲料量的特异性,需要更长的研究时间。
Atividade enzimática do solo de pastos de Brachiaria manejados sob ofertas de forragem
The soil enzymatic activity reflects the microorganism’s activity that decompose the organic matter and release nutrients to the plants. The objective was to evaluate the soil enzymatic activity in the Brachiaria pastures managed under the forage allowances of 4, 7, 10 and 13 kg MS/100 kg BW. The grazing method adopted was mob-stocking, with four grazing cycles (between December 2008 and February 2009). We also performed a sampling in June 2009 for better characterization, as the mineralization of soil organic matter occurs in the long term. The experimental design was completely randomized, with three replications and time subdivided plots. Forage allowances did not maintain a pattern of the activity of the enzyme’s dehydrogenase, protease, arylsulfatase and cellulase. Among the cv., there was a greater (P<0.05) activity of dehydrogenase and arylsulfatase in the cv. Marandu. Activity of dehydrogenase observed was 169,90; 189,19; 185,65; 247,74; and 126,00 μg TFF/g soil, and arylsulfatase was 29,69; 31,19; 38,14; 34,22; and 38,33 μg paranitrofenol/g soil, respectively to 1, 21, 42, 63, and 183 evaluation days. The protease activity was higher (P<0.05) for cv. Marandu, with the exception of evaluation day 42, where the highest activity was for cv. Mulato (82,38 μg tirosina/g soil). For cv. Marandu, the protease activity obtained was 80,49; 108,23; 86,01; and 134,71 μg tirosina/g soil solo, respectively to 1, 21, 63, and 183 evaluation days. Marandu cultivar is the most indicate to promove better conditions for the microorganisms responsible to the enzymatic activity of dehydrogenase, arylsulfatase, and protease. It can be inferred that a longer study time is required in order to increase the specificity of the forage allowances in relation to the behavior of the enzyme activity.