LMO1通过调节bcl-2和bax促进肝细胞癌的发展

IF 0.1 4区 医学
Jianfeng Zhang, B. Peng, Zhuowen Li, Yuan Zhang, Yongjun Zhang, Xiushan Lu, Yanwen Cao, Zijun Dai, Chuanyin Xiong
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引用次数: 0

摘要

现有的治疗方法对肝细胞癌(HCC)没有令人满意的结果。LMO1在几种肿瘤中异常表达,但其与HCC的关系尚不清楚。因此,我们拟评价LMO1在HCC中的作用及临床意义。收集HCC患者癌组织及癌旁组织。将LMO1/对照sirna转染HepG2细胞,免疫组织化学和Western blot检测相关蛋白表达,qPCR检测LMO1 mRNA水平,CCK8检测细胞增殖,流式细胞术检测细胞凋亡。肝细胞癌组织中LMO1阳性率明显高于癌旁组织。LMO1表达与肿瘤组织TNM分期及淋巴结转移有关(P <0.05)。LMO1表达阳性患者的累积生存率低于LMO1表达阴性患者(P <0.05)。TNM分期、淋巴转移和LMO1表达是影响HCC生存的独立危险因素;转染LMO1-siRNA后,细胞增殖明显降低,细胞凋亡增加,bax上调,bcl-2下调(P < 0.05)。综上所述,LMO1可能通过调控bcl-2/bax的表达影响HCC的发生发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
LMO1 Promotes the Development of Hepatocellular Carcinoma by Regulation of bcl-2 and bax
The existing treatments have no satisfactory outcomes for hepatocellular carcinoma (HCC). LMO1 is abnormally expressed in several tumors, but its relationship with HCC is unclear. Therefore, we intend to evaluate LMO1’s role in HCC and the clinical significance. The HCC patients cancer tissues and adjacent tissues were collected. LMO1/control-siRNA was transfected into HepG2 cells followed by analysis of relevant protein expression by immunohistochemistry and Western blot, LMO1 mRNA level by qPCR, cell proliferation by CCK8 assay and apoptosis by flow cytometry. LMO1 positive rate was significantly elevated in HCC tissues relative to adjacent tissues. LMO1 expression was related to TNM stage and lymph node metastasis of tumor tissues (P <0.05). Patients with positive LMO1 level had a lower cumulative survival rate than those with negative expression (P <0.05). TNM stage, lymphatic metastasis and LMO1 expression were identified to be independent risk factors affecting HCC survival; cells transfected with LMO1-siRNA showed significantly reduced proliferation, increased cell apoptosis and upregulated bax as well as downregulated bcl-2 (P < 0.05). In conclusion, LMO1 may affect the occurrence and development of HCC through regulation of the bcl-2/bax expression.
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