抗坏血酸- 2-磷酸释放超临界二氧化碳泡沫聚(l -乳酸- co -epsilon-己内酯)支架支持尿路上皮细胞生长，促进人类脂肪来源的基质细胞增殖和胶原蛋白的产生

IF 3.1 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2023-03-04 DOI:10.1155/2023/6404468

Alma Kurki, Kaarlo Paakinaho, M. Hannula, J. Hyttinen, S. Miettinen, Reetta Sartoneva

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引用次数: 1

摘要

组织工程为尿道大面积缺损的修复提供了一种新的方法。细胞种子支架旨在防止尿道狭窄和瘢痕形成，因为有效的尿路上皮和间质组织再生在尿道修复中很重要。在这项研究中，目的是评估新型多孔抗坏血酸2-磷酸(A2P)释放超临界二氧化碳泡沫聚l -乳酸-co- α -己内酯(PLCL)支架(scPLCLA2P)对人尿路上皮细胞(hUC)和人脂肪源性基质细胞(hASC)单培养和共培养的活力、增殖、表型维持和胶原生成的影响。scPLCLA2P支架在单培养和共培养中均支持hUC生长和表型。在单次培养中，与不含A2P的scPLCL支架相比，scPLCLA2P支架上的hASCs增殖和胶原生成明显增加，hASCs在A2P支架上形成非增殖细胞团。我们的研究结果表明，释放a2p的scPLCLA2P是一种很有前途的尿道组织工程材料。

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Ascorbic Acid 2-Phosphate-Releasing Supercritical Carbon Dioxide-Foamed Poly(L-Lactide-Co-epsilon-Caprolactone) Scaffolds Support Urothelial Cell Growth and Enhance Human Adipose-Derived Stromal Cell Proliferation and Collagen Production

Tissue engineering can provide a novel approach for the reconstruction of large urethral defects, which currently lacks optimal repair methods. Cell-seeded scaffolds aim to prevent urethral stricture and scarring, as effective urothelium and stromal tissue regeneration is important in urethral repair. In this study, the aim was to evaluate the effect of the novel porous ascorbic acid 2-phosphate (A2P)-releasing supercritical carbon dioxide-foamed poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds (scPLCLA2P) on the viability, proliferation, phenotype maintenance, and collagen production of human urothelial cell (hUC) and human adipose-derived stromal cell (hASC) mono- and cocultures. The scPLCLA2P scaffold supported hUC growth and phenotype both in monoculture and in coculture. In monocultures, the proliferation and collagen production of hASCs were significantly increased on the scPLCLA2P compared to scPLCL scaffolds without A2P, on which the hASCs formed nonproliferating cell clusters. Our findings suggest the A2P-releasing scPLCLA2P to be a promising material for urethral tissue engineering.

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来源期刊

Journal of Tissue Engineering and Regenerative Medicine 生物-工程：生物医学

CiteScore

7.50

自引率

3.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Tissue Engineering and Regenerative Medicine publishes rapidly and rigorously peer-reviewed research papers, reviews, clinical case reports, perspectives, and short communications on topics relevant to the development of therapeutic approaches which combine stem or progenitor cells, biomaterials and scaffolds, growth factors and other bioactive agents, and their respective constructs. All papers should deal with research that has a direct or potential impact on the development of novel clinical approaches for the regeneration or repair of tissues and organs. The journal is multidisciplinary, covering the combination of the principles of life sciences and engineering in efforts to advance medicine and clinical strategies. The journal focuses on the use of cells, materials, and biochemical/mechanical factors in the development of biological functional substitutes that restore, maintain, or improve tissue or organ function. The journal publishes research on any tissue or organ and covers all key aspects of the field, including the development of new biomaterials and processing of scaffolds; the use of different types of cells (mainly stem and progenitor cells) and their culture in specific bioreactors; studies in relevant animal models; and clinical trials in human patients performed under strict regulatory and ethical frameworks. Manuscripts describing the use of advanced methods for the characterization of engineered tissues are also of special interest to the journal readership.