中性粒细胞和单核细胞胞外陷阱在covid - 19综合征诊断中的应用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
J. Salmasi, G. Poryadin, MI Panina, V. Larina, A. Ryzhikh, E. Stodelova, A. Kazimirskii
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引用次数: 0

摘要

covid -19后综合征(长covid,后covid状态)的特征是认知和精神障碍,胸部和关节疼痛,嗅觉和味觉受损,以及胃肠道和心脏疾病。新冠肺炎后综合征的诊断主要基于患者的主诉。迄今为止,还没有提出最佳的诊断方法。本研究旨在比较新冠肺炎后综合征患者常规评估中获得的指标与血液中性粒细胞(NETs)和单核细胞(METs)细胞外陷阱水平的信息价值。该研究收集了21名年龄在18-59岁的后冠状病毒综合征患者的中性粒细胞和单核细胞。采用荧光显微镜和SYBR Green (Evrogen)双链DNA荧光染料对细胞外陷阱进行计数和成像。临床和实验室指标使得无法确定后冠状病毒综合征特有的变化。同时,新冠肺炎后综合征以血管内皮炎症为特征。在血液中发现的丝状net是无菌性炎症的实验室特征。丝状NETs仅在有轻度至重度СOVID-19病史的患者中检测到,而丝状METs在有严重感染史的患者中发现。研究结果表明,血液中丝状NETs和METs的检测是covid后综合征最有信息的诊断特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neutrophil and monocyte extracellular traps in the diagnosis of post-COVID syndrome
Post-COVID syndrome (long covid, post COVID-19 condition) is characterized by cognitive and mental disorders, chest and joint pain, impaired sense of smell and taste, as well as by gastrointestinal and cardiac disorders. The diagnosis of post-COVID syndrome is based mainly on the patients' complaints. To date, no optimal diagnostic method has been proposed. The study was aimed to compare the informative value of the indicators obtained during conventional assessment of patients with post-COVID syndrome and the blood levels of neutrophil (NETs) and monocyte (METs) extracellular traps. The study involved neutropils and monocytes collected from 21 patients with post-COVID syndrome aged 18–59. Fluorescence microscopy and the SYBR Green (Evrogen) fluorescent dye for double-stranded DNA were used for enumeration and imaging of extracellular traps. Clinical and laboratory indicators make it impossible to identify the changes specific for post-COVID syndrome. At the same time, post-COVID syndrome is characterized by inflammation in the vascular endothelium. The filamentous forms of NETs found in blood are a laboratory feature of such aseptic inflammation. The filamentous forms of NETs have been detected only in those patients who have a history of mild to severe СOVID-19, while the filamentous forms of METs have been found in patients having a history of severe infection. The findings show that the detection of the filamentous forms of NETs and METs in blood is the most informative diagnostic feature of post-COVID syndrome.
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来源期刊
Bulletin of Russian State Medical University
Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-
CiteScore
0.80
自引率
0.00%
发文量
59
期刊介绍: Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.
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