脂肪酶产生菌的检测及其分子特性研究

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Alawiah M. Alhebshi, Fadwa Al-Sayied, O. El-Hamshary
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引用次数: 0

摘要

背景脂肪酶是一种具有多种应用和工业应用前景的水解酶。脂肪酶被用作生物催化剂,用于制造食品配料等产品,并用于制造精细化学品。由微生物(主要是细菌和真菌)产生的脂肪酶是生物技术应用和有机化学中应用最广泛的一类酶。微生物酶也比相应的植物和动物酶更稳定,生产更方便、更安全,这使它们在商业用途中更重要。植物油加工厂的含油环境、工业废物、被油污染的土壤和柴油污染的土壤为产脂肪酶的微生物提供了合适的栖息地。目的本研究旨在检测沙特阿拉伯吉达地区不同来源、不同地区的脂肪酶产生菌。此外,已根据形态学、生物化学和分子表征对检测到的菌株进行了鉴定。已经检测到一些分离菌株的质粒图谱。材料与方法从沙特阿拉伯吉达不同地区采集了36份被燃料和机油污染的土壤样品。用吐温20培养基检测菌株的脂肪分解活性。本研究分离的细菌通过形态学和生化测试以及16SrRNA进行鉴定。结果与讨论结果表明,53株菌株为阳性菌株,能产生脂肪酶,15株菌株被筛选为强脂肪酶产生菌。序列以登录号提交给NCBI GenBank。使用质粒分离小型制剂从显示脂肪酶产生的选定菌株中分离质粒。结果表明,分离株FS6和FS15没有质粒,而FS8只有一个质粒(≈1295.5bp)。此外,分离株FS10和FS11具有两个质粒(≈1539.3和1295.5bp)。此外,分离物FS9具有三个质粒(≈1539.3、1295.5和417.7bp)。分离物具有较强的脂肪酶活性,是生产脂肪酶的良好来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and molecular characterization of lipase-producing bacteria
Background Lipase is a type of hydrolytic enzyme that has several applications and industrial efforts. Lipases are used as biological catalysts to manufacture products such as food ingredients and applied in making fine chemicals. The type of lipase produced from microbes, mainly from bacteria and fungi, represents the most widely used class of enzymes in biotechnological applications and organic chemistry. Microbial enzymes are also more stable than their corresponding plant and animal enzymes, and their production is more convenient and safer, which makes them more important in commercial uses. The oily environment of vegetable oil-processing factories, industrial wastes, soil contaminated with oil, and diesel fuel-polluted soil provides a suitable habitat for lipase-producing microorganisms. Objective This study aims to detect new strains of lipase-producing bacteria from diverse sources and different areas in Jeddah, Saudi Arabia. Furthermore, the detected bacterial strains have been identified based on morphological, biochemical, and molecular characterization. The plasmid profile of some isolated bacterial strains has been detected. Materials and methods A total of 36 soil samples contaminated with fuel and engine oil were collected from different areas in Jeddah, Saudi Arabia. Tween 20 medium was used to detect the lipolytic activity of the bacterial strains. The isolated bacteria in this study were identified by morphological and biochemical tests and 16SrRNA. Results and discussion Results showed that 53 isolates were positive and able to produce lipase, and 15 isolates have been selected as strong lipase-producing bacteria. The sequences were submitted to the NCBI GenBank under accession numbers, accession numbers, ON360988.1 for Acinetobacter sp. (FS5), ON360990.1 for Alcaligenes faecalis (FS8), ON360991.1 for Acinetobacter baumannii (FS9), ON360992.1 for Bacillus tropicus (FS10), ON360993.1 for A. baumannii (FS11), ON360994.1 for Sphingomonas aeria (FS15), and ON360996.1 for A. baumannii (FS17). Plasmids were isolated from selected strains that showed lipase production using a plasmid-isolation miniprep. Results indicated that isolates FS6 and FS15 have no plasmids, whereas FS8 has one plasmid (≈1295.5 bp). Furthermore, isolates FS10 and FS11 have two plasmids (≈1539.3 and 1295.5 bp). In addition, isolate FS9 has three plasmids (≈1539.3, 1295.5, and 417.7 bp). The isolates showed strong lipase activity and could be good sources for the production of lipase.
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
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0.00%
发文量
37
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