共沉淀法合成nHAP/壳聚糖和nHAP/纤维素复合支架骨组织工程细胞毒性研究

IF 0.5 Q4 ENGINEERING, BIOMEDICAL

Journal of Biomimetics, Biomaterials and Biomedical Engineering Pub Date : 2023-05-31 DOI:10.4028/p-14k07c

M. Shokrzadeh, F. Motafeghi, N. Farhadyar, F. Fathi

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引用次数: 1

摘要

由于纳米羟基磷灰石/纤维素(nHAP/Cel)和纳米羟基磷灰石/壳聚糖(nHAP/CS)具有良好的生物相容性、细胞毒性和力学性能，本研究的主要目的是利用共沉淀法合成纳米羟基磷灰石/纤维素(nHAP/Cel)和纳米羟基磷灰石/壳聚糖(nHAP/CS)支架用于骨组织工程。采用红外吸收光谱(FT-IR)、x射线衍射(XRD)和扫描电镜(SEM)对支架进行表征。采用3-(4,5 -二甲基噻唑-2-基)- 2,5 -二苯基-四唑溴化铵(MTT)法在骨髓细胞中评价这些纳米颗粒的细胞毒性。支架的孔隙率估计为87%。结果表明，纳米复合支架具有良好的形态、组织生物相容性和生物降解性，可用于组织工程。

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Cytotoxicity Study of nHAP/Chitosan and nHAP/Cellulose Composite Scaffolds Synthesized via Co-Precipitation for Bone Tissue Engineering

The main purpose of this study is to synthesize nano-hydroxyapatite/cellulose (nHAP/Cel) and nano-hydroxyapatite/chitosan (nHAP/CS) scaffolds via co-precipitation method for bone tissue engineering due to their suitable biocompatibility, cytotoxicity and mechanical properties. The characterizations of these scaffolds were investigated by Infrared absorption spectra (FT-IR), X-ray Diffraction (XRD), and Scanning Electron Microscope (SEM). The cytotoxicity of these nanoparticles was evaluated with bone marrow cell using the 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazoliumbromide) (MTT) assay. The porosity of scaffolds was estimated 87%. The results indicate that the nano composite scaffolds have good morphology, tissue biocompatibility and biodegradability to be used for tissue engineering.

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来源期刊

Journal of Biomimetics, Biomaterials and Biomedical Engineering ENGINEERING, BIOMEDICAL-

CiteScore

1.40

自引率

14.30%

发文量