结构优化的电纺支架用于生物材料控制的协同增强缺陷骨愈合

Q1 Engineering
Jeong In Kim , Thi Thu Trang Kieu , Sung-Ho Kook , Jeong-Chae Lee
{"title":"结构优化的电纺支架用于生物材料控制的协同增强缺陷骨愈合","authors":"Jeong In Kim ,&nbsp;Thi Thu Trang Kieu ,&nbsp;Sung-Ho Kook ,&nbsp;Jeong-Chae Lee","doi":"10.1016/j.smaim.2023.05.002","DOIUrl":null,"url":null,"abstract":"<div><p>Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 603-620"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing\",\"authors\":\"Jeong In Kim ,&nbsp;Thi Thu Trang Kieu ,&nbsp;Sung-Ho Kook ,&nbsp;Jeong-Chae Lee\",\"doi\":\"10.1016/j.smaim.2023.05.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.</p></div>\",\"PeriodicalId\":22019,\"journal\":{\"name\":\"Smart Materials in Medicine\",\"volume\":\"4 \",\"pages\":\"Pages 603-620\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Smart Materials in Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590183423000170\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Smart Materials in Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590183423000170","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 1

摘要

骨修复过程与支架的纤维形态密切相关,并且可以通过与趋化和/或血管生成分子的偶联来促进骨修复。在这里,我们开发了聚己内酯(PCL)和胶原蛋白为基础的纤维支架,通过改进的静电纺丝装置表达不同的结构。我们将咖啡酸(CA)和/或软骨寡聚基质蛋白血管生成素1 (COMP-Ang1)偶联成纤维支架。CA偶联PCL/胶原支架(PCL/col/CA)在仿生和细胞矿化、成骨和趋化分子的表达以及细胞迁移方面表现出比单独PCL/col处理更大的治疗效果。在PCL/col/CA支架中,径向对称网状支架(rG-PCL/col/CA)的生物活性最强。rG-PCL/col/CA与COMP-Ang1的连接增加了细胞对血管内皮生长因子的表达。与单独的支架相比,comp - ang1连接的rG-PCL/col/CA在大鼠股骨缺损模型中形成了更多的新血管,表达了更多的趋化分子。与PCL/col/CA支架相比,comp - ang1偶联rG-PCL/col/CA支架促进股骨缺损更快、更大的愈合。总的来说,本研究表明,通过协同改善血管化、细胞迁移以及缺损区域新骨的形成和成熟,径向网格状纤维支架与CA和COMP-Ang1的耦合极大地增强了支架介导的骨愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing

Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing

Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信