结构优化的电纺支架用于生物材料控制的协同增强缺陷骨愈合

Q1 Engineering
Jeong In Kim , Thi Thu Trang Kieu , Sung-Ho Kook , Jeong-Chae Lee
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引用次数: 1

摘要

骨修复过程与支架的纤维形态密切相关,并且可以通过与趋化和/或血管生成分子的偶联来促进骨修复。在这里,我们开发了聚己内酯(PCL)和胶原蛋白为基础的纤维支架,通过改进的静电纺丝装置表达不同的结构。我们将咖啡酸(CA)和/或软骨寡聚基质蛋白血管生成素1 (COMP-Ang1)偶联成纤维支架。CA偶联PCL/胶原支架(PCL/col/CA)在仿生和细胞矿化、成骨和趋化分子的表达以及细胞迁移方面表现出比单独PCL/col处理更大的治疗效果。在PCL/col/CA支架中,径向对称网状支架(rG-PCL/col/CA)的生物活性最强。rG-PCL/col/CA与COMP-Ang1的连接增加了细胞对血管内皮生长因子的表达。与单独的支架相比,comp - ang1连接的rG-PCL/col/CA在大鼠股骨缺损模型中形成了更多的新血管,表达了更多的趋化分子。与PCL/col/CA支架相比,comp - ang1偶联rG-PCL/col/CA支架促进股骨缺损更快、更大的愈合。总的来说,本研究表明,通过协同改善血管化、细胞迁移以及缺损区域新骨的形成和成熟,径向网格状纤维支架与CA和COMP-Ang1的耦合极大地增强了支架介导的骨愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing

Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing

Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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