视神经炎患者的免疫状态及外周血T细胞对神经递质的敏感性

Q4 Medicine
Наталя Храменко, Людмила Величко, Наталія Коновалова, Олександра Богданова, Тетяна Серебріна, Юлія Журавок
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引用次数: 0

摘要

视神经炎(ON)是一种视神经炎症,可导致视觉功能损害。研究免疫细胞亚群和基因的表达模式对于揭示它们在早期正在进行的ON病理过程中的作用和影响具有重要意义。目的:探讨ON及其后遗症患者外周血T细胞对肾上腺素和乙酰胆碱神经递质的免疫状态和敏感性。材料和方法:研究队列包括45例接受检查的特发性ON患者:1组27例原发性急性ON患者;2、ON术后部分视神经萎缩(POA)患者9例;第3,9组为ON术后后极结构丧失。第4组(对照组)27名健康志愿者。淋巴细胞对肾上腺素和乙酰胆碱的特异性反应性使用我们的复杂方法(结合平行抽样方法)进行评估,以评估个人对药物(生物调节剂)的敏感性,该方法是在斐拉托夫眼病和组织治疗研究所免疫学实验室开发的。方法包括从个体获得淋巴细胞,用免疫组织化学检查的药物培养淋巴细胞,并使用单克隆抗体的过氧化物酶抗过氧化物酶方法。结果:急性特发性ON患者显示细胞免疫活性增加,与对照组相比,CD3、CD4和CD8细胞的绝对数量增加。急性ON患者CD4:CD8比值与正常值无显著差异。我们注意到,与对照组相比,急性ON患者体液免疫成分活性水平升高(B淋巴细胞绝对数量增加,IgA和IgM水平升高)。与急性ON相比,СD3和СD4 T细胞绝对数量的减少和正常化是ON后POA患者和ON后极结构丧失患者细胞介导免疫的特征。ON后POA患者的CD8细胞百分比低于对照组。正是这些免疫活性细胞比例的特征反映在ON组中CD4:CD8比例的增加,这与细胞毒性T细胞水平的降低有关。1组、2组和3组患者外周血对肾上腺素敏感的T细胞绝对数量增加,分别是对照组的3.4倍、2.4倍和1.7倍。此外,1组和2组患者外周血对乙酰胆碱敏感的T细胞绝对数量增加,分别是对照组的2.8倍和2.6倍。我们发现,在ON患者及其后遗症中,对肾上腺素和乙酰胆碱敏感的T细胞水平与白细胞计数和CD3、CD8、CD19和CD16淋巴细胞亚群计数有显著的直接相关性。结论:急性ON组部分细胞免疫成分(CD3、CD4、CD8)及体液免疫成分(B淋巴细胞、IgA、IgM)水平较对照组升高。ON的偏侧性仅影响总患者淋巴细胞的相对数量、CD8的比例和CD4:CD8的比值。与对照组相比,患者外周血中对肾上腺素和乙酰胆碱敏感的T细胞的绝对数量增加。ON及其后遗症患者肾上腺素、乙酰胆碱敏感T细胞水平与白细胞计数及CD3、CD8、CD19、CD16淋巴细胞亚群计数有显著的直接相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunologic status and sensitivity of peripheral blood T cells to neurotransmitters in patients with optic neuritis
Background: Optic neuritis (ON) is an optic nerve inflammation that can result in impairment of visual function. Studying expression patterns of immune cell subpopulations and genes is of importance for revealing the role and influence of each of them in the early ongoing ON pathological process. Purpose: To examine the immunologic status and sensitivity of peripheral blood T cells to adrenaline and acetylcholine neurotransmitters in patients with ON and its sequelae. Material and Methods: The study cohort included 45 patients with idiopathic ON who underwent an examination: group 1, 27 patients with primary acute ON; group 2, 9 patients with partial optic atrophy (POA) following ON; and group 3, 9 patients with loss of posterior pole structure following ON. Group 4 (controls) was composed of 27 healthy volunteers. The specific reactivity of lymphocytes to adrenaline and acetylcholine was assessed using our complex methodology (in conjunction with a parallel sampling method) for assessing the individual’s sensitivity to medicaments (biological regulators) which has been developed at Immunology laboratory, Filatov Institute of Eye Diseases and Tissue Therapy. The methodology involves obtaining lymphocytes from an individual, culturing lymphocytes with examined drugs immunohistochemically, and use of a peroxidase anti-peroxidase method with monoclonal antibodies Results: Patients with acute idiopathic ON showed increased cell immunity activity, with increased absolute numbers of CD3, CD4, and CD8 cells compared to the control group. The CD4:CD8 ratio for acute ON was not significantly different from the norm. We noted increased levels of activity of humoral immunity components (increased absolute numbers of B lymphocytes and increased IgA and IgM levels) in acute ON compared to the control group. A reduction in and normalization of absolute numbers of СD3 and СD4 T cells compared to acute ON were characteristic features of cell-mediated immunity in patients with POA following ON and those with loss of posterior pole structure following ON. The percentage of CD8 cells in patients with POA following ON was lower than in controls. It is these characteristic proportions of immunocompetent cell ratios that mirrored in increased CD4:CD8 ratios in ON groups, which is associated with a reduced level of cytotoxic T cells. Patients in groups 1, 2 and 3 had increased absolute numbers of peripheral blood T cells sensitive to adrenaline, being 3.4-fold, 2.4-fold, and 1.7-fold more than controls, respectively. In addition, patients in groups 1 and 2 had increased absolute numbers of peripheral blood T cells sensitive to acetylcholine, being 2.8-fold and 2.6-fold more than controls, respectively. We found significant direct correlations of the level of the T cells sensitive to adrenaline and acetylcholine with the leukocyte cell count and CD3, CD8, CD19 and CD16 lymphocyte subset counts in patients with ON and its sequelae. Conclusion: The levels of some cell-mediated immunity components (CD3, CD4, and CD8) and humoral immunity components (B lymphocytes, IgA and IgM) were increased in acute ON group compared to controls. The laterality of ON exerted an impact only on the relative number of lymphocytes, proportion of CD8 and CD4:CD8 ratio in total patients. Patients had increased absolute numbers of peripheral blood T cells sensitive to adrenaline and acetylcholine compared to controls. There were significant direct correlations of the level of the T cells sensitive to adrenaline and acetylcholine with the leukocyte cell count and CD3, CD8, CD19 and CD16 lymphocyte subset counts in patients with ON and its sequelae.
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来源期刊
Oftalmologicheskii zhurnal
Oftalmologicheskii zhurnal Medicine-Ophthalmology
CiteScore
0.40
自引率
0.00%
发文量
71
期刊介绍: Journal of Ophthalmology (Ukraine) contains articles on eye diseases diagnosis and treatment, eye care, eye diseases prevention, history of ophthalmology, organization of eye care to population, technical equipment problems. It is committed to publishing original scientific researches and review articles on theory and practice of Ukrainian and foreign ophthalmology, cases, reviews, inventions, historical and medical reviews. It contains peer-reviews of books on ophthalmology, articles on activity of ophthalmologic societies, congresses and conferences chronicles. Journal is designed to ophthalmologists and scientific researchers in the field of vision physiology and eye diseases clinic.
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