敲除蚊子CYP4C21可减少埃及伊蚊细胞中的登革热病毒和寨卡病毒复制

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Biosafety and Health Pub Date : 2023-06-01 DOI:10.1016/j.bsheal.2023.04.005

Xiaoxue Xie , Di Wang , Bo Li , Manjin Li , Dan Xing , Teng Zhao , Xinyu Zhou , Chunxiao Li

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引用次数: 0

摘要

埃及伊蚊(伊蚊)埃及伊蚊是登革热病毒(DENV)和寨卡病毒(ZIKV)的主要媒介。了解蚊媒与虫媒病毒之间复杂的相互作用机制对阻断病毒传播至关重要。本研究构建了CYP4C21敲除(KO) Aag2细胞(Ae。证实CYP4C21 KO可减少Aag2细胞中DENV2和ZIKV拷贝数，提示CYP4C21可能在蚊虫感染虫媒病毒过程中发挥重要作用。此外，这是首次报道与病毒感染相关的CYP4家族，为探索CYP4C21在Ae相互作用中的作用奠定了基础。并为细胞色素家族蛋白的功能提供了新的见解。

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Mosquito CYP4C21 knockout reduces dengue virus and Zika virus replication in Aedes aegypti cells

Aedes aegypti (Ae. aegypti) is a major vector of dengue virus (DENV) and Zika virus (ZIKV). Understanding the complex interaction mechanisms between mosquito vectors and arboviruses is essential to interrupt virus transmission. This study constructed CYP4C21 knockout (KO) Aag2 cells (Ae. aegypti cells) and confirmed that CYP4C21 KO reduced DENV2 and ZIKV copies in Aag2 cells, which suggests that CYP4C21 may play an important role in mosquito infection with arboviruses. Furthermore, it is the first report of the CYP4 family related to viral infection, which lays the foundation for exploring the role of the CYP4C21 in the interaction of Ae. aegypti and arbovirus and provides novel insights into the function of cytochrome family proteins.

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来源期刊

Biosafety and Health Medicine-Infectious Diseases

CiteScore

7.60

自引率

0.00%

发文量

116

审稿时长

66 days