利用二次离子质谱成像和细胞电化学技术对单个神经细胞进行化学成像和分析

IF 2.8 4区 医学 Q2 NEUROSCIENCES
Frontiers in Synaptic Neuroscience Pub Date : 2022-05-16 eCollection Date: 2022-01-01 DOI:10.3389/fnsyn.2022.854957
Alicia A Lork, Kim L L Vo, Nhu T N Phan
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引用次数: 0

摘要

神经细胞是神经系统中神经元交流的单位,是一种异质性的分子结构,它是高度介导的,以适应细胞功能。在单细胞水平上理解神经通讯的复杂调控机制需要具有高灵敏度、特异性和空间分辨率的分析技术。本文将介绍神经细胞化学成像和分析的挑战性技术。次级离子质谱(SIMS)允许在亚细胞分辨率下对神经细胞和突触进行非靶向和靶向分子成像。细胞电化学非常适合于定量从活的神经细胞释放的活性化学物质的量。这些技术也将讨论最近被证明有利于理解神经系统结构和功能关系的多模态成像方法。本文综述了这些技术在突触和神经元分析中的优势、局限性和潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry.

A nerve cell is a unit of neuronal communication in the nervous system and is a heterogeneous molecular structure, which is highly mediated to accommodate cellular functions. Understanding the complex regulatory mechanisms of neural communication at the single cell level requires analytical techniques with high sensitivity, specificity, and spatial resolution. Challenging technologies for chemical imaging and analysis of nerve cells will be described in this review. Secondary ion mass spectrometry (SIMS) allows for non-targeted and targeted molecular imaging of nerve cells and synapses at subcellular resolution. Cellular electrochemistry is well-suited for quantifying the amount of reactive chemicals released from living nerve cells. These techniques will also be discussed regarding multimodal imaging approaches that have recently been shown to be advantageous for the understanding of structural and functional relationships in the nervous system. This review aims to provide an insight into the strengths, limitations, and potentials of these technologies for synaptic and neuronal analyses.

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来源期刊
CiteScore
7.10
自引率
2.70%
发文量
74
审稿时长
14 weeks
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