口服硒可改善雄鹿解冻后精子质量

IF 0.5 Q4 REPRODUCTIVE BIOLOGY
K. Lukusa, J. Kabuba
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引用次数: 0

摘要

目的:探讨口服补硒及采精方法对萨能雄鹿解冻后精液质量的影响。方法:将16只健康雄鹿分为两组,每组8只。治疗组每隔10天接受一次硒治疗,为期3个月。冷冻保存前后每周评估精子运动参数、形态学参数、线粒体膜电位、质膜功能和精子DNA完整性。结果:人工阴道法采精时,补硒组精液形态异常的平均百分比显著降低(P<0.05),电射精法采精后补硒组近端液滴缺陷的发生率显著降低(P<0.05)补硒雄鹿经人工阴道获得精液时进行性运动能力明显高于电射精组和对照组(P<0.05),人工阴道采精时,硒处理雄鹿的精子头侧移位幅度显著高于人工阴道采精液(P<0.05)对照组(P<0.05)。人工阴道采硒样品的体外受精潜力分别显著高于电射精法和对照组(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks
Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.
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来源期刊
Asian Pacific Journal of Reproduction
Asian Pacific Journal of Reproduction Veterinary-Veterinary (all)
CiteScore
1.70
自引率
0.00%
发文量
588
审稿时长
9 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.
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