野鼠毒液的蛋白质组学和毒理学分析及抗蛇毒血清的中和作用

IF 3.6 Q2 TOXICOLOGY
Gianni Mena , Stephanie Chaves-Araya , Johelen Chacón , Enikő Török , Ferenc Török , Fabián Bonilla , Mahmood Sasa , José María Gutiérrez , Bruno Lomonte , Julián Fernández
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引用次数: 6

摘要

珊瑚蛇属于蛇科,拥有对人类致命的毒液,可以根据三指毒素(3FTxs)或磷脂酶A2 (PLA2s)的优势进行分组。对珊瑚蛇的毒液进行了蛋白质组学和毒理学分析。该种分布在哥斯达黎加东南部,以前被认为是M. alleni的一个亚种。结果表明,该毒液中含有丰富的pla2,与之前研究的Micrurus alleni毒液不同。根据蛋白质组学数据,该毒液的毒理学评估显示,它在合成底物上具有明显高于M. alleni的体外PLA2活性。用组织学评价和血浆肌酸激酶释放对CD-1小鼠的体内肌毒性评价也表明,斑胸草毒引起肌肉损伤。一种用黑尾马蛇毒制备的商业马抗蛇毒血清通过酶免疫测定显示出对雅氏马和黑尾马毒液相似的识别能力。该抗蛇毒血清也免疫识别的主要部分的叶氏分枝杆菌毒液,并能够中和其致命作用的小鼠模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Proteomic and toxicological analysis of the venom of Micrurus yatesi and its neutralization by an antivenom

Proteomic and toxicological analysis of the venom of Micrurus yatesi and its neutralization by an antivenom

Coralsnakes belong to the family Elapidae and possess venoms which are lethal to humans and can be grouped based on the predominance of either three finger toxins (3FTxs) or phospholipases A2 (PLA2s). A proteomic and toxicological analysis of the venom of the coralsnake Micrurus yatesi was performed. This species, distributed in southeastern Costa Rica, was formerly considered a subspecies of M. alleni. Results showed that this venom is PLA2-rich, in contrast with the previously studied venom of Micrurus alleni. Toxicological evaluation of the venom, in accordance with proteomic data, revealed that it has a markedly higher in vitro PLA2 activity upon a synthetic substrate than M. alleni. The evaluation of in vivo myotoxicity in CD-1 mice using histological evaluation and plasma creatine kinase release also showed that M. yatesi venom caused muscle damage. A commercial equine antivenom prepared using the venom of Micrurus nigrocinctus displayed a similar recognition of the venoms of M. yatesi and M. nigrocinctus by enzyme immunoassay. This antivenom also immunorecognized the main fractions of the venom of M. yatesi and was able to neutralize its lethal effect in a murine model.

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来源期刊
Toxicon: X
Toxicon: X Pharmacology, Toxicology and Pharmaceutics-Toxicology
CiteScore
6.50
自引率
0.00%
发文量
33
审稿时长
14 weeks
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