剪接因子调节机械生长因子的产生调节成骨细胞在机械刺激下的生理过程

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2019-01-01 DOI:10.1080/19336918.2019.1686103

Qian Yi, Huan Liu, Jianguo Feng, Yanjiao Wu, Weichao Sun, Mengting Ou, Liling Tang

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引用次数: 4

摘要

机械刺激影响成骨细胞的各种生理过程。我们之前的研究表明，机械生长因子(MGF)是胰岛素样生长因子1的剪接变体，在机械刺激下成骨细胞中高度表达。本研究旨在探讨MGF在成骨细胞中的系统功能，以及机械应力调控Igf1选择性剪接生成MGF的机制。我们发现MGF促进成骨细胞的增殖和迁移，同时通过Erk1/2途径抑制成骨细胞的分化。此外，循环拉伸上调ASF/SF2的表达，进而调节MGF的表达。我们的研究结果表明，机械刺激通过增加MGF的表达来影响成骨细胞的生理反应，而MGF的表达受剪接因子的调节。

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Splicing factor-modulated generation of mechano growth factor regulates physiological processes in osteoblasts under mechanical stimuli

ABSTRACT Mechanical stimuli influence various physiological processes in osteoblasts. We previously showed that mechano-growth factor (MGF), a splicing variant of insulin-like growth factor 1, is highly expressed in osteoblasts in response to mechanical stimuli. This study aims to explore the systemic functions of MGF in osteoblasts, and the mechanisms by which mechanical stress regulates the alternative splicing of Igf1 to generate MGF. We found that MGF promoted the proliferation and migration of osteoblasts, while it inhibited their differentiation via Erk1/2 pathway. Furthermore, cyclic stretching upregulated the expression of ASF/SF2, which in turn regulated the expression of MGF. Our findings indicate that mechanical stimuli influence the physiological responses of osteoblasts by increasing the expression of MGF, which is regulated by splicing factors.

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.