Fei Liao, Taotao Bao, Guangyao Tao, Yanchun Hu, Changquan Han
{"title":"荨麻叶乙酸乙酯提取物对疥螨的体外杀螨效果及成分评价","authors":"Fei Liao, Taotao Bao, Guangyao Tao, Yanchun Hu, Changquan Han","doi":"10.17221/6/2023-VETMED","DOIUrl":null,"url":null,"abstract":"<p><p>In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of <i>Urtica fissa</i> leaf ethyl acetate extract against <i>Sarcoptes scabiei</i> mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all <i>S. scabiei</i> within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against <i>S. scabiei</i> were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for <i>S. scabiei</i> was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(<i>S</i>)-HpOTrE (4.624%), nandrolone (4.264%), 1<i>H</i>-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that <i>Urtica fissa</i> contains promising new acaricidal compounds capable of successfully controlling animal mites.</p>","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"1 1","pages":"200-207"},"PeriodicalIF":0.6000,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581513/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>In vitro</i> evaluation of the composition and acaricidal efficacy of <i>Urtica fissa</i> leaf ethyl acetate extract against <i>Sarcoptes scabiei</i> mites.\",\"authors\":\"Fei Liao, Taotao Bao, Guangyao Tao, Yanchun Hu, Changquan Han\",\"doi\":\"10.17221/6/2023-VETMED\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of <i>Urtica fissa</i> leaf ethyl acetate extract against <i>Sarcoptes scabiei</i> mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all <i>S. scabiei</i> within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against <i>S. scabiei</i> were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for <i>S. scabiei</i> was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(<i>S</i>)-HpOTrE (4.624%), nandrolone (4.264%), 1<i>H</i>-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that <i>Urtica fissa</i> contains promising new acaricidal compounds capable of successfully controlling animal mites.</p>\",\"PeriodicalId\":23532,\"journal\":{\"name\":\"Veterinarni Medicina\",\"volume\":\"1 1\",\"pages\":\"200-207\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581513/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinarni Medicina\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.17221/6/2023-VETMED\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/5/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinarni Medicina","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17221/6/2023-VETMED","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/5/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites.
In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites.
期刊介绍:
The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.