荨麻叶乙酸乙酯提取物对疥螨的体外杀螨效果及成分评价

IF 0.6 4区 农林科学 Q3 VETERINARY SCIENCES
Veterinarni Medicina Pub Date : 2023-05-24 eCollection Date: 2023-05-01 DOI:10.17221/6/2023-VETMED
Fei Liao, Taotao Bao, Guangyao Tao, Yanchun Hu, Changquan Han
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引用次数: 0

摘要

在兽医学中,天然产品为螨虫管理提供了化学药剂的替代品。本研究考察了荨麻叶乙酸乙酯提取物对疥螨的杀螨效果。采用液相色谱-质谱(LC-MS)分析方法测定提取物的化学成分。发现乙酸乙酯提取物在浓度为100 mg/ml (m/v)时对螨虫极具毒性,可在2小时内杀死所有疥螨。25、50和100 mg/ml乙酸乙酯提取物对金黄色葡萄球菌的中位致死时间(LT50)分别为1.706、1.204和0.750 h。2 h的中位致死剂量(LC50)为19.14 mg/ml。采用LC-MS对乙酸乙酯提取物的化学成分进行鉴定,主要成分为schaftoside(8.259%)、carnosol(6.736%)、前列腺素A2(5.94%)、13(S)-HpOTrE(4.624%)、诺龙酮(4.264%)、1h -吲哚-3-甲醛(4.138%)、9-oxoODE(3.206%)和硬脂酸(2.891%)。综上所述,这些发现表明,荨麻含有有希望的新型杀螨化合物,能够成功地控制动物螨。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites.

In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites.

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来源期刊
Veterinarni Medicina
Veterinarni Medicina Veterinary Sciences-兽医学
CiteScore
1.30
自引率
0.00%
发文量
62
审稿时长
18-36 weeks
期刊介绍: The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.
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