用含脯氨酸肽评价外伤性脑损伤模型Wistar大鼠脑的一些参数

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
Anna A. Kolesnikova, M. Y. Fleishman, Yulia B. Malofey, N. Yakusheva, I. V. Tolstenok
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引用次数: 0

摘要

目的——在创伤性脑损伤(TBI)模型上,检测Wistar大鼠在给予含脯氨酸肽(也称为甘氨酰脯氨酸)(Arg-Gly-Arg-Pro-Gly-Pro[RGPRPP]和Thr-Lys-Pro-Arg-Pro-Gly-Pro[WKPRPGP;Selank])后神经元的形态计量学参数和脑组织的氧化状态。研究对象——我们的研究涉及26只成年雄性Wistar大鼠(2.5-3个月大,220-300克)。研究了神经元的形态计量学参数和动物脑组织的氧化状态。材料和方法——组成四个实验组。第1组包括完整的对照动物。在实验的第二天,通过50克重量的动物从50厘米的高度自由下落对其他三组的动物进行TBI,并接受以下注射:第2组为0.9%氯化钠溶液;第3组中的WKPRPGP肽溶液;第4组RGRPGP肽溶液。从实验的第1天到第5天,每天以0.1mg/kg的剂量腹膜内给药物质。在苏木精和伊红染色的石蜡切片上研究了大鼠脑神经元的形态计量学参数。用化学发光法研究了脑组织中自由基过程的强度。结果——形态计量学参数分析显示,与相同条件下的WKPRGP肽相比,TBI后引入RGRPGP肽后,神经元细胞质面积、核仁面积、核仁数量和细胞核核仁指数显著增加。TBI和肽给药后,我们在Wistar大鼠的新皮层中观察到氧化应激,在RGRPGP治疗的动物组中更为明显。结论:RGRPGP肽给药后,我们观察到闭合性TBI模型中神经元的形态计量学参数增加:更大的面积和更多的核仁。化学发光数据表明,WKPRRPGP肽能更好地保护大鼠脑组织免受TBI引起的氧化应激的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluating Some Parameters Of Wistar Rat Brain In Traumatic Brain Injury Model With Administration Of Proline-Containing Peptides
Objective — To examine the morphometric parameters of neurons and the oxidative status of the Wistar rat brain tissues after administering proline-containing peptides, also known as glyprolines (Arg-Gly-Arg-Pro-Gly-Pro [RGRPGP] and Thr-Lys-Pro-Arg-Pro-Gly-Pro [WKPRPGP; Selank]), on a traumatic brain injury (TBI) model. Study subjects — Our study involved 26 mature male Wistar rats (2.5-3 mos. old, 220-300 g). The morphometric parameters of neurons and the oxidative status of animal brain tissues were studied. Material and Methods — Four experimental groups were formed. Group 1 included intact control animals. Animals in three other groups were subjected to TBI via free fall of a 50 g weight from a height of 50 cm on the second day of the experiment and received the following injections: 0.9% sodium chloride solution in Group 2; WKPRPGP peptide solution in Group 3; RGRPGP peptide solution in Group 4. Substances were administered intraperitoneally on a daily basis at a dose of 0.1 mg/kg from day 1 through day 5 of the experiment. Morphometric parameters of rat brain neurons were studied on paraffin sections stained with hematoxylin and eosin. The intensity of free radical processes in the brain tissue was investigated by chemiluminescence. Results — An analysis of morphometric parameters revealed significant increases in the neuronal cytoplasm area, nucleolar area, number of nucleoli, and nuclear-nucleolar index with the introduction of the RGRPGP peptide after TBI, compared with the WKPRPGP peptide under the same conditions. After TBI and peptide administration, we observed an oxidative stress in the neocortex of Wistar rats, and it was more pronounced in the group of animals treated with RGRPGP. Conclusion — After RGRPGP peptide administration, we observed an increase in the morphometric parameters of neurons in the closed TBI model: a larger area and a greater number of nucleoli. Chemiluminescence data implied that WKPRRPGP peptide better protected brain tissue in rats from the effects of oxidative stress caused by TBI.
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来源期刊
Russian Open Medical Journal
Russian Open Medical Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
0.90
自引率
0.00%
发文量
39
期刊介绍: Russian Open Medical Journal (RusOMJ) (ISSN 2304-3415) is an international peer reviewed open access e-journal. The website is updated quarterly with the RusOMJ’s latest original research, clinical studies, case reports, reviews, news, and comment articles. This Journal devoted to all field of medicine. All the RusOMJ’s articles are published in full on www.romj.org with open access and no limits on word counts. Our mission is to lead the debate on health and to engage, inform, and stimulate doctors, researchers, and other health professionals in ways that will improve outcomes for patients. The RusOMJ team is based mainly in Saratov (Russia), although we also have editors elsewhere in Russian and in other countries.
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