血清和胸水促肽-1和缺氧诱导因子-1α浓度在评估恶性胸腔积液患者淋巴结受累中的临床意义。

Zeliha Cansel Ozmen, Mustafa Kupeli
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引用次数: 0

摘要

简介:恶性胸腔积液(MPE)和淋巴结转移(LNM)是预后不良的因素,对癌症患者具有重要意义。本研究的目的是确定胸水(P)和血清(S)中的缺氧诱导因子-1α(HIF-1α)和促敏素-1(CD133)是否可以作为诊断MPE患者淋巴结受累的生物标志物。材料和方法:56名MPE患者和30名健康对照受试者。应用计算机断层扫描(CT)和正电子发射断层扫描(PET)对胸腔积液进行诊断。在胸水细胞学检查中有恶性细胞的患者被纳入MPE组。35名CT上有淋巴结转移的患者被纳入LNM阳性MPE组。采用酶联免疫吸附法(ELISA)测定MPE患者血清和胸水HIF-1α和CD133的浓度。结果发现,淋巴结转移阳性的恶性患者组CD133/HIF-1α(S)比值高于淋巴结转移阴性组,而HIF-1α浓度较低。胸水HIF-1α和CD133/HIF-1α(S)比值对MPE患者的淋巴结转移有足够的诊断作用(AUC=0.90和0.83)。根据本实验,HIF-1α(P)和CD133/HIF-1α比率的浓度可作为诊断MPE患者淋巴结受累的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Clinical importance of serum and pleural fluid prominin-1 and hypoxia-inducible factor-1α concentration in the evaluation of lymph node involvement in patients with malignant pleural effusion.

Clinical importance of serum and pleural fluid prominin-1 and hypoxia-inducible factor-1α concentration in the evaluation of lymph node involvement in patients with malignant pleural effusion.

Introduction: Malignant pleural effusion (MPE) and lymph node metastasis (LNM) presence are poor prognostic factors that have importance for cancer patients. The study objective was to determine whether hypoxia-inducible factor-1α (HIF-1α) and prominin-1 (CD133) in pleural fluid (P) and serum (S) could be used as biomarkers for diagnosis of lymph node involvement in patients with MPE.

Materials and methods: Fifty-six patients with MPE and 30 healthy control subjects were included. Computerized tomography (CT) and positron emission tomography (PET) were used to diagnose pleural effusion. Patients with malignant cells in pleural fluid cytological examination were included in the MPE group. Thirty-five patients with lymph node metastases on CT were included in the LNM-positive MPE group. Serum and pleural fluid HIF-1α and CD-133 concentrations were measured manually via enzyme-linked immunosorbent assay (ELISA).

Results: Serum concentrations of HIF-1α and CD133 were higher in MPE patients. It was found that CD133/HIF-1α (S) ratio was higher in the malignant patient group with positive lymph node involvement than in the negative group, while concentrations of HIF-1α (P) were lower. Pleural fluid HIF-1α and CD133/HIF-1α (S) ratio had sufficient performance in diagnosing lymphatic metastases in patients with MPE (AUC = 0.90 and 0.83, respectively).

Conclusions: In conclusion, serum HIF-1α and CD133 concentrations were higher in patients with MPE, consistent with our hypothesis. Concentrations of HIF-1α (P) and CD133/HIF-1α (S) ratio can be used as biomarkers in diagnosing lymph node involvement in MPE patients, according to this experiment.

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